FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828173
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTACTGTGCGAAATGGGATCGCAGCAGCTGGACGGGCCCCTTTGA35320.42648094057642544No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGGTCATTTGCAAATACA34720.4192360774862257No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTG21080.25453618990235133No Hit
CTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAACCT20910.2524834786934614No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC20140.24318590439437168No Hit
CTCCATAATCAGTAGTACGATCCCACACCTGACAGTAATAGTCGGCCTCA19570.23630328445868196No Hit
TTATGATACCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGTTCCA18440.22265879230547242No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17690.21360271344272272No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCCATAATCAGTA16920.20430513914363302No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC16470.19887149182598324No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16040.1936793399446734No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14780.17846512745525392No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14430.17423895731930406No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14400.17387671416479408No Hit
GCCGTATATTACTGTGCGAAATGGGATCGCAGCAGCTGGACGGGCCCCTT14390.17375596644662408No Hit
GTCTGTGACCTCCTTTGTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCC14130.1706165257742042No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCC13910.16796007597446427No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13730.16578661704740436No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13280.16035296972975452No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13040.15745502449367463No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13020.15721352905733463No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC12090.145983991267525No Hit
AAGTAATAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGG12080.14586324354935504No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGGTCATTTGCAAATACAGTA11900.1436897846222951No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT11840.14296529831327512No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11800.14248230744059515No Hit
GGTATAAGTGGTGGTGGTGGCAGCACTTACTACGCAGACTCCGTGAAGGG11440.13813538958647528No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11270.13608267837758536No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11050.13342622857784545No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA11030.13318473314150547No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10960.13233949911431547No Hit
ATTATGGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC10840.13089052649627553No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCGTCAACTATGCCATGAGCTGGG10840.13089052649627553No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT10750.12980379703274558No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACTCCATAATCAGTAG10580.12775108582385566No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA10510.12690585179666566No Hit
ATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC10380.12533613146045572No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA10320.12461164515143576No Hit
GGGTCTGTGACCTCCTTTGTGCTGACTCAGCCACCCTCAGTGTCAGTGGC10110.12207594306986584No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT10100.12195519535169584No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAACCTTGGTACGCCCTGGGG9900.11954024098829594No Hit
GTATCATAATGGATGACCACCACAGGGGCCTGGCCTGGCTTCTGCTGGTA9890.11941949327012594No Hit
CAGTAGTACGATCCCACACCTGACAGTAATAGTCGGCCTCATCCCCGGCT9880.11929874555195594No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9870.11917799783378594No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9830.11869500696110595No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG9760.11784977293391598No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9710.11724603434306599No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9600.11591780944319603No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT9560.11543481857051606No Hit
GCACAGGGTCTGTGACCTCCTTTGTGCTGACTCAGCCACCCTCAGTGTCA9420.11374435051613613No Hit
CCCTCAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCGTCAACTATGCC9350.11289911648894614No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9270.11193313474358618No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT9200.11108790071639621No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT8970.10831070319848632No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCGGCCA8970.10831070319848632No Hit
CCCCAGGACAGACGGCCAGGATCACCTGTGGGGGAAACAACATTGGAAGT8950.10806920776214632No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT8940.10794846004397632No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGTTCCAACTCTGGGAACACGG8830.10662023514410636No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC8800.10625799198959637No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT8790.10613724427142637No Hit
GATCAGGACTGAACAGAAAGAACTCACCATGGAGTTTGGGCTGAGCTGGC8740.1055335056805764No Hit
GATTATGGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCC8730.1054127579624064No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8720.1052920102442364No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8700.10505051480789643No Hit
CCTCTCTCACTGCACAGGGTCTGTGACCTCCTTTGTGCTGACTCAGCCAC8640.10432602849887644No Hit
ATATTACTGTGCGAAATGGGATCGCAGCAGCTGGACGGGCCCCTTTGACT8600.10384303762619647No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8520.10287705588083648No Hit
GTACTACTGATTATGGAGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTA8490.10251481272632651No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8310.10034135379926656No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)