FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625187
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG16320.2610418962646376No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT12360.19770084790630646No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG10860.1737080265584537No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9760.1561132909033617No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG9540.15259434377234332No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT8170.13068090027463783No Hit
ATATATGGCCGTGTCCGCGGCGGTCACAGAATTCAGATTCAGGGAGAATT7970.12748185742825746No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT7630.12204348458941085No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA7500.11996410673926361No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC7390.1182046331737544No Hit
CTACTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGACATCAAGCGA7390.1182046331737544No Hit
GTCTTATATGATGGGAGGACCACCTACAACCCGTCCCTCAATAGTCGAGT7300.11676506389288324No Hit
ATAATATATGGCCGTGTCCGCGGCGGTCACAGAATTCAGATTCAGGGAGA7200.11516554246969307No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6820.10908736106157038No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6690.10700798321142316No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG6450.10316913179576671No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6410.10252932322649064No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6260.10013004109170535No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)