FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930681
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTGAGATCTCCACTAGCACTAATACTTGAGACCCACTCCAGCCCCT19560.21016868293217547No Hit
GAAATAGACGGCCGTGTCCTCGACTCTCAGGCTGTTCATTTGCAAAAACA15520.16675960936131715No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT12720.13667411282705888No Hit
CTCACATACTACGCAGACTCCGTGAAGGGCCGGTTCACCCTCTCCAGAGA12470.13398790777935726No Hit
TCTCTGGAGAGGGTGAACCGGCCCTTCACGGAGTCTGCGTAGTATGTGAG11790.12668143004960883No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11730.12603674083816044No Hit
CTCCAGAGACAATTCCATGAACACGCTGTTTTTGCAAATGAACAGCCTGA11560.12421012140572334No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA11240.12077177894466525No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11050.11873026310841202No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG10870.11679619547406683No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC10820.11625895446452653No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC10740.115399368849262No Hit
GCCTGGGGGATCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTA10310.11077909616721518No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10010.10755565010997323No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9810.10540668607181194No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA9720.10443965225463935No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9690.10411730764891515No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT9620.10336517023555869No Hit
GTGTGTACCTAGCATGCAGTAATAAAGTCCAACATCCTCAGCCTCCACCC9600.10315027383174255No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)