FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1450966
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCTCAGTTTTCAGACTGTTCATTTGCAGATACA42110.29022044624064247No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT40490.27905547063128977No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA38500.26534046972844294No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAACAGTCT35970.2479038102891453No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGCCTCTG35560.2450781065855437No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCGCCTTCCATAATGCGTGG34840.24011589520360918No Hit
GTCTATTACTGTGTCACAGATCTCGGCAAGCTCCCAGAGAAGATCACGGG32990.22736576873613853No Hit
GTACTATCCCACACCTGACAGAAATAGTCGGCCTCATCCCCGGCCTCGAC32190.2218522005339891No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31660.21819946160006506No Hit
GTACTAGTCATCAAAGACTGTTCGGCGGGGGGACCAAGCTGACCGTCCTG30810.21234129538528126No Hit
GACTAGTACTATCCCACACCTGACAGAAATAGTCGGCCTCATCCCCGGCC30090.20737908400334676No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT29990.20668988797807805No Hit
CCTATGTGCTGACTCAGTCTCCCTCCGAGTCCGTGGCCCCAGGAAACACG29710.20476013910732577No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT29060.2002803649430793No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC27950.1926302890625969No Hit
ATCCCACACCTGACAGAAATAGTCGGCCTCATCCCCGGCCTCGACCCTGC26990.18601400722001757No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT25220.17381523757276188No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24110.1661651616922795No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA24020.16554488526953767No Hit
GTCTTTGATGACTAGTACTATCCCACACCTGACAGAAATAGTCGGCCTCA22980.15837724660674338No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG22900.15782588978652842No Hit
CCCTGAATACTGGGGCCAGGGAAGCCACGTCACCGTCTCGTCAGCCTCCA22510.15513802528798057No Hit
GGATAGTACTAGTCATCAAAGACTGTTCGGCGGGGGGACCAAGCTGACCG22090.1522434019818521No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT21840.15052041191868037No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG21340.14707443179233698No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA20340.14018247153965013No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA20320.1400446323345964No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG20170.13901083829669336No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18940.13053372718588857No Hit
GTATTCAGGGAGGTCGGTATATTCTCCCGTGATCTTCTCTGGGAGCTTGC18780.1294310135454587No Hit
GCATTACCTGTGGGGGAATACACATTGGAAGAGACGGTGTGAACTGGTAC18760.12929317434040494No Hit
GCCTCTGTGACCTCCTATGTGCTGACTCAGTCTCCCTCCGAGTCCGTGGC18740.1291553351353512No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC18640.12846613911008253No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC18440.12708774705954518No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC18370.12660530984185708No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG18330.1263296314317496No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA18290.12605395302164213No Hit
CTCCTGTGCAGCCTCTGGATTCGCCTTCCATAATGCGTGGATGAGCTGGG17820.12281473170287932No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAAGTCTGGGAATATGG17620.12143633965234196No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA17150.11819711833357914No Hit
TCATACAACAGTCTGTATCTGCAAATGAACAGTCTGAAAACTGAGGACAC16960.11688764588556864No Hit
GCTTATGGTCAGGGTGGCCATATTCCCAGACTTGGAGCCAGAGAATCGCT16860.11619844986029997No Hit
GTATTATGATCGGGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCT16770.11557817343755815No Hit
CCTCTCTCACTGCACAGCCTCTGTGACCTCCTATGTGCTGACTCAGTCTC16540.11399302257944018No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT16310.11240787172132222No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGACCTGGA16240.11192543450363414No Hit
GTCCTGGTGCACGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA15920.10972000722277435No Hit
CTACAAGACCACGCCTCCCATGCTGGACTCCGACGGCTCCTTCTTCCTCT15830.10909973080003253No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15780.10875513278739818No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA15730.10841053477476385No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT15650.1078591779545489No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG15460.1065497055065384No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT15450.10648078590401154No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA15410.10620510749390408No Hit
ACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAACAGTCTTTGATGACTAG15370.10592942908379659No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15290.10537807226358165No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15230.10496455464842043No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA15090.10399968021304429No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA14860.1024145293549263No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14840.10227669014987258No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC14840.10227669014987258No Hit
GACCCAGGACGGTCAGCTTGGTCCCCCCGCCGAACAGTCTTTGATGACTA14790.10193209213723822No Hit
GTCTGAAAACTGAGGACACGGCCGTCTATTACTGTGTCACAGATCTCGGC14770.10179425293218448No Hit
GTGTGGGATAGTACTAGTCATCAAAGACTGTTCGGCGGGGGGACCAAGCT14750.10165641372713076No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC14590.10055370008670086No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)