FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026564
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAATAGCCGTGTCTGCGGCGGTCACAGAGCTCAAGTTCAGGAAGA46290.4509217155481782No Hit
TAGTAATACAGCCCGCTCGCACAGTAATAAATAGCCGTGTCTGCGGCGGT42000.4091318222731364No Hit
ATGTATGACAGTGGGACAACCTACTACAACCCGTCCCTCAAGAGTCGAGT35890.34961288336625873No Hit
ATTTATTACTGTGCGAGCGGGCTGTATTACTATGATAGTGATGGTTATTA35070.3416250715980689No Hit
GTATTACTATGATAGTGATGGTTATTACCTACCAAACTTTGACTACTGGG32360.31522632782758797No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTGGACAC23230.226288862652499No Hit
GTCATACATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA18330.1785568167206331No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18200.17729045631835913No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC17190.16745181011607654No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16760.1632630795547087No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16180.15761316391379399No Hit
ATATAATAGTTATTCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATGA15770.15361925802969909No Hit
CTATCATAGTAATACAGCCCGCTCGCACAGTAATAAATAGCCGTGTCTGC15640.15235289762742507No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15500.15098912488651464No Hit
GTCTCTGCTGACAGTAGTATTTACTACTGGGGCTGGATCCGCCAGCCCCC15350.1495279398069677No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG14690.14309872545696128No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14410.14037117997514037No Hit
CTATGATAGTGATGGTTATTACCTACCAAACTTTGACTACTGGGGCCAGG13930.13569538772059025No Hit
GTAATAACCATCACTATCATAGTAATACAGCCCGCTCGCACAGTAATAAA13680.1332600792546787No Hit
ATTTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTG13400.1305325337728578No Hit
GTAGTAGGTTGTCCCACTGTCATACATACTCCCAATCCACTCCAGCCCCT13150.12809722530694628No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13040.1270256895819452No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12610.12283695902057738No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12270.1195249395069377No Hit
GGTTATTACCTACCAAACTTTGACTACTGGGGCCAGGGAACCCTGGTCAC11600.11299831281829482No Hit
CTATTATATCGCAGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCT11570.11270607580238542No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTGGACACGTCG11310.11017335499783744No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT10880.10598462443646961No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10780.10501050105010501No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG10360.10091918282737365No Hit
GTAATACAGCCCGCTCGCACAGTAATAAATAGCCGTGTCTGCGGCGGTCA10310.10043212113419134No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)