FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026564
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAATAGCCGTGTCTGCGGCGGTCACAGAGCTCAAGTTCAGGAAGA46520.4531621993368168No Hit
TAGTAATACAGCCCGCTCGCACAGTAATAAATAGCCGTGTCTGCGGCGGT40190.39150018897993694No Hit
ATGTATGACAGTGGGACAACCTACTACAACCCGTCCCTCAAGAGTCGAGT35530.3461060391753461No Hit
ATTTATTACTGTGCGAGCGGGCTGTATTACTATGATAGTGATGGTTATTA34960.3405535358730678No Hit
GTATTACTATGATAGTGATGGTTATTACCTACCAAACTTTGACTACTGGG32750.31902540903440996No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTGGACAC22860.22268460612294996No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21490.20933911572975478No Hit
GTCATACATACTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGA19190.18693427784336875No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17250.16803628414789532No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC16620.16189930681379827No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15630.15225548528878863No Hit
CTATCATAGTAATACAGCCCGCTCGCACAGTAATAAATAGCCGTGTCTGC15500.15098912488651464No Hit
GTCTCTGCTGACAGTAGTATTTACTACTGGGGCTGGATCCGCCAGCCCCC15350.1495279398069677No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15310.14913829045242186No Hit
ATATAATAGTTATTCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATGA14890.1450469722296905No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14370.13998153062059454No Hit
GTAATAACCATCACTATCATAGTAATACAGCCCGCTCGCACAGTAATAAA13970.13608503707513608No Hit
CTATGATAGTGATGGTTATTACCTACCAAACTTTGACTACTGGGGCCAGG13590.13238336820695057No Hit
GTAGTAGGTTGTCCCACTGTCATACATACTCCCAATCCACTCCAGCCCCT13590.13238336820695057No Hit
ATTTACTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTG13260.12916876103194735No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13210.12868169933876505No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12700.12371367006830554No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12590.12264213434330447No Hit
CTATTATATCGCAGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCT12230.11913529015239185No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG11850.11543362128420634No Hit
GGTTATTACCTACCAAACTTTGACTACTGGGGCCAGGGAACCCTGGTCAC11840.11533620894556987No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA11570.11270607580238542No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11450.11153712773874791No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT11160.10871216991829052No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCACCATGTCCGTGGACACGTCG11060.10773804653192592No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10280.10013988411828195No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG553.3318525E-452.360669
GTATCAA16750.035.6999361
ATCAACG16700.034.5075533
TCAACGC16850.034.2003634
CTACCGT850.00283675333.8820766
GGCAGCG4700.033.7018557
CAACGCA17750.032.466265
TATCAAC18850.032.100262
TATCGCA6100.031.8685957
GTACACG9300.031.7571545
CAGCGCT5750.031.3025689
TACACGG9450.031.2378436
CTGTGCG22950.030.4296449
AACGCAG19000.030.3155446
GAATACG1902.2076347E-730.3140669
ACACGGG9850.029.9693037
CGCAGAG19150.029.7021088
CAGCCCG23800.029.6453749
GTACATG26650.028.9261571
GTAATAC25450.028.5873743
Produced by FastQC (version 0.11.5)