FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1033682
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATAATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCC36150.34972070714204173No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT34910.3377247548085388No Hit
GTATTATGATTGGCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAGTCA31750.3071544246683216No Hit
GTAATAAACGGCCGTGTCGGCGGCGGTCACAGAGGTCAGATTCAGGGAGA29280.28325926155239234No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC23410.22647197107040656No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22250.21524995114551673No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19460.18825905839513504No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT19320.18690467668006217No Hit
GTCTGGGACAGAGTTCACTCTCACCATCAGCGGCCTGCAGTCTGAAGATT18890.18274478998376678No Hit
GTTTATTACTGTGCGAGAGCCGGAAACTGGAACTTCCTCGGGTTCGACCC18400.17800445398101156No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGACACCAGTGGCCCTGGT18060.17471524124440593No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17020.1646541199324357No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16470.1593333346232207No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16070.15546367258015523No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15870.15352884155862248No Hit
GTATTCCCTGGCCCCAGGGGTCGAACCCGAGGAAGTTCCAGTTTCCGGCT15180.14685367453433457No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG14630.14153288922511953No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG14390.13921109199928025No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13790.13340659893468204No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13470.13031086930022967No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGTAGGGACTTAGTCTGGTACCAGC13290.1285695213808502No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13090.12663469035931746No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA13080.12653794880824082No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC13060.12634446570608757No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT13040.1261509826039343No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12910.12489334243993801No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12910.12489334243993801No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12610.12199109590763892No Hit
TTATTACTGTCAGCAGTATTATGATTGGCCTCGGACGTTCGGCCAAGGGA12570.12160412970333237No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC12440.12034648953933608No Hit
TTCTGAGACTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTC12150.11754098455811363No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA12100.11705727680273043No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG12020.11628334439411733No Hit
GAGTTCACTCTCACCATCAGCGGCCTGCAGTCTGAAGATTTTGCAGTTTA11730.11347783941289488No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11700.11318761475966498No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11550.11173649149351542No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA11540.11163974994243878No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11450.11076907598274906No Hit
TCCCTAAAGAGTCGAATTAGCATGTCAATAGACACATCCAAGAATCAATT11360.10989840202305931No Hit
CTCCCAGGCTCCTCATGTATGGTGTTTCCACCAGGGCCACTGGTGTCCCA11330.1096081773698294No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11280.10912446961444623No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA11080.10718963859291349No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA11070.10709289704183686No Hit
CCTCATGTATGGTGTTTCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCA11050.10689941393968358No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAACGTC10880.10525480757138075No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA10680.10331997654984802No Hit
CTCATGGACCTCCTGCACAAGAACATGAAACACCTGTGGTTCTTCCTCCT10470.10128840397723865No Hit
CTTTAGGGAGGCGTTGTAGTAGGTGCCCCCACTGGTATGGATGCGCCCAA10420.10080469622185546No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)