FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences771995
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG26130.3384736947778159No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG22080.2860122151050201No Hit
GTAATATACGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATACA17920.23212585573740763No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG17430.2257786643695879No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT14850.1923587588002513No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT14160.1834208770782194No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC13970.18095972124171789No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG13530.1752602024624512No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT12710.16463837201018142No Hit
CCTCATATATGGTGCATCCAGCAGGGCCACGGGCATCCCAGACAGGTTCA12270.1589388532309147No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT11960.1549232831818859No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT10660.138083795879507No Hit
GTATATTACTGCGCAAGAAGGGTCCCGTACAGCAGCTCGTCCGCCTATTT10460.13549310552529484No Hit
TATATGAGGAGCCTGGGAGCCTGGCCAGGCTTCTGCTGGTACCAGGCTAA10330.13380915679505695No Hit
ATATACGGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATACAGCG10220.1323842771002403No Hit
GTTAGGACCCAGCGGGAACCATGGCAACCCCAGCGCAGCTTCTCTTCCTC9880.12798010349807964No Hit
GTCCCCAGGCACCCTGTCTGTGTCTCCAGGGGAGAGAGCCACCCTCTCCT9720.12590755121470995No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9030.11696966949267806No Hit
CATATATGGTGCATCCAGCAGGGCCACGGGCATCCCAGACAGGTTCAGTG8530.1104929436071477No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8400.10880899487690981No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC8290.10738411518209315No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT8150.10557063193414465No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC7960.10310947609764312No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT7880.10207319995595826No Hit
CTATATGAGACCTCATTGTGGACGTTCGGCCACGGGACCAAGGTGGAAAT7800.10103692381427341No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7730.10013018219029916No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT806.1583523E-754.0011676
ATCAACG15400.050.033543
GTATCAA16450.049.044571
TCAACGC15700.048.612514
CAACGCA16000.047.701035
TATCAAC16950.046.307882
AACGCAG17200.044.7916646
ATAGGTG10200.043.765654
GTCATAG10700.043.75911
CATAGGT10750.042.2017253
CAGAGCG9150.041.7031179
TAGGTGG10600.041.434865
CGCAGAG18650.040.9204838
GGTATCA8800.038.472821
GTACATG19600.038.2222371
GGTGGTC12300.038.0471347
TACATGG18650.037.4533842
TCATAGG12500.036.869572
GTGGTCA12200.036.5885858
GCAGAGT21100.036.1690529
Produced by FastQC (version 0.11.5)