FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505500
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA21370.4227497527200791No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG17990.3558852621167161No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15760.31177052423343227No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG11960.23659742828882296No Hit
GTGTATTACTGTGCGAGATGTGGCGGTGGTAGCTGGCCCGATTACTACTA11100.21958456973293772No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT10070.19920870425321463No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT9680.1914935707220574No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG9520.18832838773491592No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG9070.1794263105835806No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC8730.17270029673590506No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT7940.15707220573689418No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7880.15588526211671613No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT7750.1533135509396637No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC6760.13372898120672602No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCCACTCAGGCTGC6210.1228486646884273No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC6010.11889218595450049No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG5970.11810089020771514No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC5960.11790306627101879No Hit
AATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGA5890.11651829871414442No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACTCCACTCAGGCTG5830.11533135509396637No Hit
GTATTTATTACTTCCATCATATGATATAACTGCCACCCACTCCAGCCCCT5740.11355093966369931No Hit
CCCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATACCGT5720.11315529179030663No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA5680.11236399604352128No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA5680.11236399604352128No Hit
CAGTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATA5620.11117705242334322No Hit
ATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACAGCG5560.10999010880316518No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT5410.10702274975272008No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC5300.10484668644906033No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACT5280.10445103857566766No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG5240.1036597428288823No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT5210.10306627101879326No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC5090.10069238377843719No Hit
GGGCTGGAGTGGGTGGCAGTTATATCATATGATGGAAGTAATAAATACTA5060.10009891196834818No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACG300.001987763272.011733
CGAACGT400.006217913753.9981044
ATAGGCG657.588693E-444.306145
TATACTG2200.042.5439645
CAGAGCG6500.042.090839
ATCAACG18200.039.566883
TCAACGC18200.039.559054
TATCAAC18250.039.4584772
CAACGCA18250.039.4506725
AACGCAG18700.038.5013286
GTATCAA20500.037.2463871
GGACGGG800.00210635735.9987376
CGCAGAG20250.035.554318
GCACACA7650.034.82922
ATGTCGT1102.5359052E-432.7261286
GCAGAGT22800.031.893629
TCTACAC2757.2759576E-1231.4232983
TGTAGAC1153.2876348E-431.3094443
ATAGGTG8350.030.1785834
TAGGTGG8650.029.964275
Produced by FastQC (version 0.11.5)