FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864468
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTAGTCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCA39510.4570441011118977No Hit
GTATATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA35030.4052203204745578No Hit
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA31420.3634605329520584No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT30770.35594145763637286No Hit
GCTCATATACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCA29580.34217576590457943No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22940.26536551960280774No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT19380.22418412248920724No Hit
CTCCTAAGCTGCTCATATACTGGGCATCTACCCGGGAATCCGGGGTCCCT16510.19098451301841132No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16220.1876298486467978No Hit
TCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG15860.18346543770272583No Hit
GTATAAAACACTCTGGCTGGACCTGCAGTTGATGGTGGCCCTCTCGCCCA15770.18242433496670785No Hit
CTCATATACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAG15460.17883831443153478No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15460.17883831443153478No Hit
TTATTACTGTCAGCAATATTATAGTAGTCCTTACACTTTTGGCCAGGGGA14840.17166627336118861No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14160.16380016380016382No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13820.1598671090196514No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13470.15581837615735922No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13230.15304210219464456No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTA13200.15269506794930524No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12190.14101158168954778No Hit
TTATAGTAGTCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC11530.13337682829208253No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11170.12921241734801056No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11060.127939958448433No Hit
GTTTATTACTGTCAGCAATATTATAGTAGTCCTTACACTTTTGGCCAGGG10590.12250308860478351No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG10520.1216933420323251No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10110.11695054067935423No Hit
ATAGTAGTCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA9910.1146369790437587No Hit
GTGTAAGGACTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTC9890.11440562288019915No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC9380.10850604070943054No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA9090.10515137633781702No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA8980.10387891743823946No Hit
ACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGC8970.10376323935645969No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8930.1033005270293406No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)