FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864468
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTAGTCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCA40550.4690746216169945No Hit
GTATATGAGCAGCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTA34750.40198133418472404No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT29710.34367958096771656No Hit
CTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGCT29260.33847406728762663No Hit
GTTTTATACAGCTCCAACAATAAGAACTACTTAGCTTGGTACCAGCAGAA23200.26837314972908194No Hit
GCTCATATACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCA22440.2595816155138189No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22180.2565739853875447No Hit
GTATAAAACACTCTGGCTGGACCTGCAGTTGATGGTGGCCCTCTCGCCCA16010.18520060892942247No Hit
TCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG15830.1831184034573865No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15630.180804841821791No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14910.17247601993364706No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14390.16646075968109866No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14150.16368448571838404No Hit
GTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGTGA13990.16183363640990758No Hit
GTGTAAGGACTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTC13840.16009846518321094No Hit
GCTTAGGAGGCTGTCCTGGTTTCTGCTGGTACCAAGCTAAGTAGTTCTTA13510.1562810884844783No Hit
TTATTACTGTCAGCAATATTATAGTAGTCCTTACACTTTTGGCCAGGGGA13490.15604973232091876No Hit
CTCCTAAGCTGCTCATATACTGGGCATCTACCCGGGAATCCGGGGTCCCT13350.1544302391760019No Hit
ACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAGGC13270.15350481452176368No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13230.15304210219464456No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12970.15003447206837037No Hit
CTCATATACTGGGCATCTACCCGGGAATCCGGGGTCCCTGACCGATTCAG12730.14725819810565574No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11680.13511199951877917No Hit
TTATAGTAGTCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC11440.13233572555606454No Hit
GTTTATTACTGTCAGCAATATTATAGTAGTCCTTACACTTTTGGCCAGGG11340.13117894473826677No Hit
CAGTAATAAACTGCCACATCTTCAGCCTGCAGGCTGCTGATGGTGAGAGT10550.1220403762776644No Hit
ATAGTAGTCCTTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGA10020.11590943794333623No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9720.11243909548994295No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA9560.11058824618146651No Hit
CTACTATAATATTGCTGACAGTAATAAACTGCCACATCTTCAGCCTGCAG9350.10815900646409121No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9320.10781197221875188No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA9300.10758061605519233No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC9260.10711790372807321No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA9200.10642383523739457No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9120.10549841058315634No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9010.10422595168357879No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG13150.050.9390073
GTATCAA13600.050.8541221
TCAACGC13250.050.554564
CAACGCA14500.046.1964075
TATCAAC14750.045.9097062
ACGCAGA14750.045.8831447
GAATACG2750.044.504969
ACCGTAC657.591689E-444.3061458
AACGCAG15300.043.763186
CGCAGAG15350.043.620638
TTTCGCG907.7973105E-539.9962849
GTGCTAG4500.032.019261
GTACATG23250.028.8173371
ATATTAT31200.028.6326121
ACATGGG23050.028.1232053
GCAGAGT24100.028.0803819
ATTATAG28200.027.840093
CATTAGC8100.027.5545928
TTATAGT28500.027.5470374
TATAGTA28500.027.2943125
Produced by FastQC (version 0.11.5)