FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences685598
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTCTTCATTTGCAGATACA33320.48599908401133024No Hit
GTATATGTAATTACCACTACTAGTAATGGATGCGACCCACTCCAGACCCT32820.47870618058979164No Hit
CTCCTAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTG22890.3338691186380357No Hit
GTCTATTACTGTGTAAGACAACGGGGTGTTTTGACTGGTCAAGAGATCAC22840.33313982829588185No Hit
TTACATATACTATGCAGACTCACTGAAGGGCCGATTCACCATCTCCAGAG22510.3283265120376664No Hit
TCCTAGGAGTTTGTAGACCTTGCATGCAGTAATACACCCCAACATCCTCA22250.32453420225846635No Hit
TACATATACTATGCAGACTCACTGAAGGGCCGATTCACCATCTCCAGAGA21700.3165120084947739No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT20280.2958001627776044No Hit
GTATTACTGCATGCAAGGTCTACAAACTCCTAGGACGTTCGGCCAAGGGA18290.2667744071598809No Hit
ATACTATGCAGACTCACTGAAGGGCCGATTCACCATCTCCAGAGACAACG17010.2481045744007421No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15190.22155840594634174No Hit
GTCTTACACAGTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTCTTCATT14510.21164005729304927No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT13200.19253265032861822No Hit
GTGTAAGACAACGGGGTGTTTTGACTGGTCAAGAGATCACCCCACTCTAC13030.1900530631652951No Hit
GCATAGTATATGTAATTACCACTACTAGTAATGGATGCGACCCACTCCAG12580.18348945008591042No Hit
GTCTACAAACTCCTAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA12300.1794054241698488No Hit
CAGTAATACACCCCAACATCCTCAGCCTCCACTCTGCTGATCTTCAGTGT12230.1783844176908334No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12040.17561311439064875No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG10890.15883943652111004No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10870.1585477203842485No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA10780.15723499776837155No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGG10720.1563598493577869No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCACTGGG10410.151838249236433No Hit
GTAATACACCCCAACATCCTCAGCCTCCACTCTGCTGATCTTCAGTGTAA10350.15096310082584838No Hit
GTAGTAGAGTGGGGTGATCTCTTGACCAGTCAAAACACCCCGTTGTCTTA10190.14862937173095603No Hit
CTCCAGACCCTTCCCTGGAGCCCGGCGGACCCAGTTCATGCTATAGGTAC9930.144837061951756No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC9870.14396191354117135No Hit
GTAATGGAAAGAATTATTTGGATTGGTACCTGCAGAAACCAGGGCAGTCT9840.14352433933587905No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9530.13900273921452513No Hit
CTCCTACATAGTAATGGAAAGAATTATTTGGATTGGTACCTGCAGAAACC9520.13885688114609435No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9400.13710658432492512No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9390.13696072625649433No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGT8950.13054297124554037No Hit
GGTCTGGAGTGGGTCGCATCCATTACTAGTAGTGGTAATTACATATACTA8840.1289385324928019No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8820.12864681635594036No Hit
AAGTAGTAGAGTGGGGTGATCTCTTGACCAGTCAAAACACCCCGTTGTCT8600.1254379388504634No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8540.12456279043987876No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG8530.12441693237144799No Hit
GTGTATTACTGCATGCAAGGTCTACAAACTCCTAGGACGTTCGGCCAAGG8380.12222906134498643No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT8240.12018704838695562No Hit
ATGTAGGAGGCTCTGAGTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAG8160.11902018383950945No Hit
ACCTTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC8140.1187284677026479No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7950.11595716440246326No Hit
GTACAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7870.11479029985501708No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT7790.11362343530757091No Hit
TTTAGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAATCTGGGGGAGGCC7770.11333171917070936No Hit
ATGCAGACTCACTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAG7720.11260242882855552No Hit
CCTACATAGTAATGGAAAGAATTATTTGGATTGGTACCTGCAGAAACCAG7590.11070627393895548No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTACCTATAGC7530.10983112552837085No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTACCTATAGCATGAACTGGG7330.10691396415975542No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7330.10691396415975542No Hit
ACTCAGAGCCTCCTACATAGTAATGGAAAGAATTATTTGGATTGGTACCT7270.1060388157491708No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTACTCAGAGCCTCCTA7110.10370508665427844No Hit
CTACTACTTCTACTACGGCATGGACGTCTGGGGCCGGGGGACCACGGTCA7020.1023923640384015No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)