FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences685598
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATGTAATTACCACTACTAGTAATGGATGCGACCCACTCCAGACCCT34550.5039396264283151No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTCTTCATTTGCAGATACA31040.45274344440911435No Hit
TACATATACTATGCAGACTCACTGAAGGGCCGATTCACCATCTCCAGAGA23450.3420371704701589No Hit
GTCTATTACTGTGTAAGACAACGGGGTGTTTTGACTGGTCAAGAGATCAC22900.33401497670646646No Hit
TTACATATACTATGCAGACTCACTGAAGGGCCGATTCACCATCTCCAGAG22850.3332856863643126No Hit
CTCCTAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTG22430.3271596474902202No Hit
TCCTAGGAGTTTGTAGACCTTGCATGCAGTAATACACCCCAACATCCTCA20910.304989221088743No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT20430.29798803380406597No Hit
GTATTACTGCATGCAAGGTCTACAAACTCCTAGGACGTTCGGCCAAGGGA17160.2502924454272037No Hit
ATACTATGCAGACTCACTGAAGGGCCGATTCACCATCTCCAGAGACAACG16980.2476670001954498No Hit
GTCTTACACAGTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTCTTCATT14560.21236934763520315No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14460.21091076695089542No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT12910.18830276634412585No Hit
GTCTACAAACTCCTAGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA12780.18640661145452583No Hit
GTGTAAGACAACGGGGTGTTTTGACTGGTCAAGAGATCACCCCACTCTAC12770.18626075338609505No Hit
GCATAGTATATGTAATTACCACTACTAGTAATGGATGCGACCCACTCCAG12390.18071814678572573No Hit
CAGTAATACACCCCAACATCCTCAGCCTCCACTCTGCTGATCTTCAGTGT11910.17371695950104873No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11130.16234003016344856No Hit
GTAATACACCCCAACATCCTCAGCCTCCACTCTGCTGATCTTCAGTGTAA11010.16058973334227927No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10980.160152159136987No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGG10850.15825600424738695No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC10420.15198410730486378No Hit
CTCCTACATAGTAATGGAAAGAATTATTTGGATTGGTACCTGCAGAAACC10400.15169239116800223No Hit
CTCCAGACCCTTCCCTGGAGCCCGGCGGACCCAGTTCATGCTATAGGTAC10040.14644150070449446No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA10030.1462956426360637No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG9910.14454534581489442No Hit
GTAATGGAAAGAATTATTTGGATTGGTACCTGCAGAAACCAGGGCAGTCT9910.14454534581489442No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCACTGGG9910.14454534581489442No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9740.14206575865157134No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9680.1411906102409867No Hit
GTAGTAGAGTGGGGTGATCTCTTGACCAGTCAAAACACCCCGTTGTCTTA9670.14104475217255594No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC9560.13944031341981744No Hit
TCATAAGGGTATCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT8720.12718823567163265No Hit
GTGTATTACTGCATGCAAGGTCTACAAACTCCTAGGACGTTCGGCCAAGG8630.1258755130557557No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8520.12427107430301723No Hit
AAGTAGTAGAGTGGGGTGATCTCTTGACCAGTCAAAACACCCCGTTGTCT8470.12354178396086336No Hit
ACCTTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC8340.12164562907126333No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8260.12047876452381716No Hit
GGTCTGGAGTGGGTCGCATCCATTACTAGTAGTGGTAATTACATATACTA8190.11945775804480176No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGT8060.11756160315520173No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8050.11741574508677095No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG7900.11522787406030939No Hit
CCTACATAGTAATGGAAAGAATTATTTGGATTGGTACCTGCAGAAACCAG7730.11274828689698628No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT7660.11172728041797088No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTACTCAGAGCCTCCTA7550.11012284166523238No Hit
ATGTAGGAGGCTCTGAGTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAG7500.10939355132307854No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTACCTATAGCATGAACTGGG7410.1080808287072016No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTACCTATAGC7380.10764325450190929No Hit
GTGTTGTAGTTGTTCTCCGGCTGCCCGCTGCTCTCCCACTCCACGGCGAT7370.10749739643347851No Hit
TTTAGAAGGTGTCCAGTGTGAGGTGCAACTGGTGGAATCTGGGGGAGGCC7330.10691396415975542No Hit
ATGCAGACTCACTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAG7330.10691396415975542No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA7320.10676810609132464No Hit
GATTTTACACTGAAGATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTGTA7110.10370508665427844No Hit
ACTCAGAGCCTCCTACATAGTAATGGAAAGAATTATTTGGATTGGTACCT7080.10326751244898613No Hit
GTACAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG7060.1029757963121246No Hit
CTACTACTTCTACTACGGCATGGACGTCTGGGGCCGGGGGACCACGGTCA7020.1023923640384015No Hit
CTCCAGAGACAACGCCAAGAACTCACTGTATCTGCAAATGAAGAGCCTGA6990.1019547898331092No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGT6920.1009337833540938No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12200.037.202011
GTATAGG1752.582965E-937.0501631
GTGCTAG1951.8189894E-1036.944611
GTCTATA1251.32060195E-534.5801541
GAATACG1051.9308546E-434.2831769
AGGCCGT1301.7359826E-533.2258876
TCAACGC12950.032.815034
ATCAACG13000.032.6912043
TAGGAGT12750.031.6350424
TATCAAC13950.031.4976332
GGCAGCG4350.029.7887277
CAACGCA14300.029.717115
GTATAGC1703.162244E-629.6643471
GTACACG5000.028.8104845
AACGCAG14750.028.795776
ATACTGC1753.985713E-628.7957696
CCAATAC1804.9401096E-628.0122383
CTAGGAG14250.027.8016193
CGCAGAG15300.027.7626538
GTTTGTA13250.027.7111579
Produced by FastQC (version 0.11.5)