FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280076

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280076
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743810
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTCCGGTGTTCGG12720.17101141420524058No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG11510.15474381898603137No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC10720.1441228270660518No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG10380.1395517672523897No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC10230.13753512321695058No Hit
GATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG9870.1326951775318966No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT9190.1235530579045724No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCATTCAGG8820.11857866928382248No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC7720.10378994635726867No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGG7500.1008322017719579No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC8950.047.4808464
ATCAACG9200.046.1906133
CAACGCA9550.044.494775
AACGCAG10350.041.740186
GTATCAA11100.040.9016531
ATACCGT1105.517655E-639.2737166
ACGCAGA11150.038.7453737
TATCAAC11300.037.6115762
CGCAGAG11550.036.7801488
TTAGACG850.002831117333.8946044
GGTATCA7200.029.026111
GCAGAGT16150.026.3005319
ACATGGG16900.023.4404263
GTACATG18950.023.1976491
GTATAGA4750.022.7573131
TACATGG18200.022.560642
GTATTAG4359.094947E-1121.5366131
GCCCATA1700.003201921221.1955361
CAGAGTA15250.020.77159
TTTATAC7100.020.2890243
Produced by FastQC (version 0.11.5)