FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences916992
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC23660.258017518146287No Hit
GACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCACATTGGTGTTA19520.21286990508096035No Hit
GCCCCACTCTGTGTCGGGGTCTCCGGGGAAGACGGTAACCATCTCCTGCA18610.20294615438302624No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT17750.19356766471245115No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCAC16290.17764604271356785No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15760.17186627582356226No Hit
TCTCTGGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAC15510.16913997068676717No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15270.1665227177554439No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14800.16139726409826913No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA14420.15725328029034058No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA14250.15539939279731993No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13820.1507101479620324No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT13740.14983773031825795No Hit
ACCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGC13110.14296744137353434No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA13080.14264028475711893No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC12660.1380600921273032No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12590.13729672668900056No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11700.12759108040201006No Hit
GGTACCAGCAGCGCCCGGGCAGTGCCCCCACCATTGTGATCCATGAGGAT11570.1261734017308766No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA11290.12311993997766611No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA10960.11952121719709659No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10940.11930311278615299No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA10910.11897595616973758No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT10880.11864879955332215No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10870.11853974734785037No Hit
GTGTCGGGGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCGGCAGCGG10780.11755827749860413No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG10560.11515912897822446No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10480.11428671133445004No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10340.11275998045784479No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10330.11265092825237298No Hit
CTCTTATAGTGGGGGCTACCAGAACTACACGTCCCTCAAGAGTCGAGTCT10310.11243282384142937No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC10200.11123324958123952No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10060.10970651870463427No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG10050.10959746649916247No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT9880.10774357900614183No Hit
ACCTAGGACGGTCAGCTTGGTCCCCCCGCCGAACACCACATTGGTGTTAT9880.10774357900614183No Hit
ACTATGTGCAGTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCATTGTG9850.1074164223897264No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA9840.1073073701842546No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT9740.10621684812953658No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGAGG9670.10545348269123393No Hit
GTCTCTGGTGCCTCCATCAGAACTACTAATTTCTATTGGGCCTGGATCCG9580.10447201284198772No Hit
TCCCTCAAGAGTCGAGTCTCCATTTCAGTAGACACGTCCAAGAACCAATT9570.10436296063651591No Hit
GTCTTACGATAACACCAATGTGGTGTTCGGCGGGGGGACCAAGCTGACCG9560.10425390843104411No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9400.10250907314349525No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9310.10152760329424902No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9280.10120044667783362No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9230.10065518565047459No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)