FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917049
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATCCGTCCCTCGAGAGTCGCCTCACCATCTCGATCGACACGCCCA28380.3094709224916008No Hit
GTCTCTGGTGGCTCCATCAGCAGCAGTCATTACCACTGGGTCTGGATCCG24330.26530752446161543No Hit
ATAATAAACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAAGGAGA20610.2247426255303697No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13100.14284950967723642No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13060.1424133279682983No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12690.13837864716062065No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12140.1323811486627214No Hit
GTTTATTATTGTGCGAGACACAGTCAGGGGTCTGACTCCTGGGGCCAGGG11820.1288916949912164No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA11470.12507510503800778No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG11340.12365751448395887No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11330.12354846905672434No Hit
ATCTTGGAGGCACCCTCTATAATCCGTCCCTCGAGAGTCGCCTCACCATC10970.1196228336762812No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10550.11504292573243087No Hit
ATCTACTATCTTGGAGGCACCCTCTATAATCCGTCCCTCGAGAGTCGCCT10470.11417056231455462No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC10370.1130801080422093No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC10150.11068110864304961No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT9980.10882733638006258No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA9970.10871829095282803No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22550.061.7686421
ATCAACG22500.061.4340483
TCAACGC22850.060.493044
CAACGCA23100.059.838365
AACGCAG24200.057.118436
TATCAAC24400.056.1060262
ACGCAGA27850.049.1155247
TACGCAA752.6584354E-547.995357
CGCAGAG28600.047.827538
GCAGAGT32600.041.7382859
GAATACG3700.040.86099
GTGCTAG5250.034.3667141
CAATACG1153.2947227E-431.3013134
CGCAAAA1204.228071E-429.9970959
GGTATCA16150.027.2592961
TATACTG3607.2759576E-1225.997485
CTAGGTA1959.243397E-625.8436474
GTACATG23450.025.5442141
GTGTAGA5700.025.3228441
GCTAGAG7400.024.3219663
Produced by FastQC (version 0.11.5)