FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917049
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATCCGTCCCTCGAGAGTCGCCTCACCATCTCGATCGACACGCCCA22160.24164466675172208No Hit
GTCTCTGGTGGCTCCATCAGCAGCAGTCATTACCACTGGGTCTGGATCCG21300.23226676000955238No Hit
ATAATAAACAGCCGTGTCTGCGGCGGTCACAGAGGTCAGCTTCAAGGAGA16090.1754540924203614No Hit
GTTTATTATTGTGCGAGACACAGTCAGGGGTCTGACTCCTGGGGCCAGGG10990.11984092453075026No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA10300.1123167900515676No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT10280.11209869919709851No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9470.10326601959110146No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9310.10152129275534895No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC12900.055.243874
ATCAACG12900.054.127833
CAACGCA14300.049.331995
GTATCAA14700.049.0278131
TATCAAC15500.045.9771542
AACGCAG16350.042.706366
ACGCAGA16300.041.9541177
CGCAGAG16400.041.259378
CGTACCC750.001536261838.3916973
ACCGATC800.002109123935.9922188
GCAGAGT20900.033.4090429
GGTATCA13250.029.3722861
GTCGTAC1000.00625292628.8283541
TCTACAC9950.027.4915433
GTATAGA3555.456968E-1226.3921571
GTACATG22450.026.0033041
GAATACG3358.185452E-1125.785479
CTCTACA11550.025.5529142
TACATGG22700.025.051852
CTATACA3001.841181E-823.994814
Produced by FastQC (version 0.11.5)