FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences644884
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATAGACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAGGGAGA35910.5568443316937619No Hit
GTCTATTATTGTGTGAGACAGATGGTTCAGGACGGGTGGTTCGACCCCTG25760.39945168433392675No Hit
GTCTCTGGTGCCTCCGTCAGCAGTAGAAGGCACCACTGGGGCTGGATCCG25240.39138821865637846No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT24910.3862710192840883No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA22980.3563431562885728No Hit
GTATAATAACTGGCTCTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA21830.33851049180937964No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20030.3105984952332512No Hit
GTCCTGAACCATCTGTCTCACACAATAATAGACAGCCGTGTCTGCGGCGG20020.31044342858560614No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC19990.3099782286426706No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT19290.2991235633075096No Hit
CAATAATAGACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAGGGA19020.2949367638210903No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18660.28935436450586466No Hit
GTTCAGGACGGGTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGT18610.28857903126763884No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG18570.28795876467705817No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC18350.2845472984288647No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17820.27632876610367135No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16820.26082210133915557No Hit
CACCTGCACTGTCTCTGGTGCCTCCGTCAGCAGTAGAAGGCACCACTGGG16150.25043263594693No Hit
CTATTATTGTGTGAGACAGATGGTTCAGGACGGGTGGTTCGACCCCTGGG15750.24422997004112365No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15320.23756210419238186No Hit
TTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT14050.21786863994144684No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13240.20530824148218904No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12380.19197250978470545No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC12080.18732051035535074No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11880.1842191774024476No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC11420.1770861116107703No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC11350.17600064507725421No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA11310.1753803784866736No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG11160.17305437877199623No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT10920.16933277922851242No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT10840.16809224604735115No Hit
ACTACAACCCGTCGCTCAAGAGTCGAGTCACCATATCCGAAGACACGTCC10700.16592131298031892No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA10680.16561117968502864No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG10670.16545611303738347No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC10650.16514597974209314No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10520.1631301133227061No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT10480.16250984673212548No Hit
GTCTCACACAATAATAGACAGCCGTGTCTGCGGCGGTCACAGAGTTCAGC10430.16173451349389967No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT10220.15847811389335137No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10130.15708251406454493No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10100.15661731412160948No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9980.15475651434986756No Hit
GTAGTAGGTGCTCCCACCAGAATACATACTCCCAATCCACTCCAGCCCCT9890.15336091452106115No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC9820.15227544798754505No Hit
GGATTGGGAGTATGTATTCTGGTGGGAGCACCTACTACAACCCGTCGCTC9530.1477785152058355No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG9480.1470031819676097No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA9150.14188598259531948No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG9100.1411106493570937No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG9010.13971504952828726No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8970.1390947829377066No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC8950.13878464964241632No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8930.138474516347126No Hit
GTGTGAGACAGATGGTTCAGGACGGGTGGTTCGACCCCTGGGGCCAGGGA8760.1358383833371583No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG8700.13490798345128738No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8700.13490798345128738No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC8680.13459785015599704No Hit
CACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA8670.1344427835083519No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT8610.13351238362248094No Hit
GTCCCTCACCTGCACTGTCTCTGGTGCCTCCGTCAGCAGTAGAAGGCACC8530.13227185044131967No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA8440.13087625061251326No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT8190.12699958442138432No Hit
CTCCCAATCCACTCCAGCCCCTTCCCTGGGGACTGGCGGATCCAGCCCCA8150.12637931783080367No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8140.12622425118315853No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8060.12498371800199726No Hit
TGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCT8010.12420838476377147No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC7890.12234758499202958No Hit
CTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACAC7890.12234758499202958No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTACCAGCAACTTAGCCTGGTACCAGC7800.12095198516322316No Hit
TTATTACTGTCAGCAGTATAATAACTGGCTCTGGACGTTCGGCCAAGGGA7690.11924625203912642No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT7640.11847091880090063No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC7520.11661011902915873No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA7470.11583478579093294No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG7420.11505945255270715No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC7340.1138189193715459No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA7330.11366385272390074No Hit
CCTCTATGAAGCATCCACCAGGGCCACTGGTGTCCCAGCCAGGTTCAGTG7320.11350878607625557No Hit
GAGTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTA7150.11087265306628788No Hit
GATTGGGAGTATGTATTCTGGTGGGAGCACCTACTACAACCCGTCGCTCA7130.11056251977099758No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT7090.10994225318041694No Hit
CTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGC7050.10932198658983631No Hit
GTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCA7010.10870171999925568No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA6920.10730612017044927No Hit
GCCCAGGACTGGTGAGGCCTTCGGAGACCCTGTCCCTCACCTGCACTGTC6910.1071510535228041No Hit
ACCTACTACAACCCGTCGCTCAAGAGTCGAGTCACCATATCCGAAGACAC6910.1071510535228041No Hit
CCCCAGGAGTTCAGGTGCTGGGCACGGTGGGCATGTGTGAGTTTTGTCAC6890.10684092022751378No Hit
GTTCAGCCTCAGGGAGAACTGCCTCTTGGACGTGTCTTCGGATATGGTGA6880.10668585357986862No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC6880.10668585357986862No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA6850.10622065363693316No Hit
CATGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTC6800.10544532039870737No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6730.10435985386519125No Hit
CCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGT6660.10327438733167517No Hit
TCATAGAGGAGGAGCCTGGGAGCCTGGCCAGGTTTCCGCTGGTACCAGGC6590.10218892079815906No Hit
GTCCTGTCCCAGTTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAGGCC6570.10187878750286873No Hit
CGTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG6530.1012585209122881No Hit
GTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGAC6520.10110345426464294No Hit
GTAGAAGGCACCACTGGGGCTGGATCCGCCAGTCCCCAGGGAAGGGGCTG6500.10079332096935263No Hit
GGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTT6480.10048318767406231No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6460.10017305437877198No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC6460.10017305437877198No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)