FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280088

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280088
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827952
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT35690.43106363653931634No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23160.27972636094846076No Hit
GAGTTACACTATCCCTCTCACCTTCGGCAGAGGGACCAAGGTGGAGATCA23030.27815622161671205No Hit
GTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGACT19830.23950663806597486No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19740.23841961852861035No Hit
GTGTATCTACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGGGGA18980.22924034243531027No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15890.19191933831912963No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC14950.18056602315110054No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14130.17066206736622413No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT14020.16933348793166753No Hit
GTAGATACACCATAGATCACGAGCCTAGGGGCTTGCCCTGGTTTCTGCTG13910.16800490849711094No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12170.14698919744139755No Hit
GTAGGAGACAGAGTCACTTTCACTTGCCGGGCAAGTCAGAACATTTACAG12130.14650607764701334No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA12050.1455398380582449No Hit
GTCTGGGACTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA11620.1403463002686146No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11580.13986318047423038No Hit
ACACTATCCCTCTCACCTTCGGCAGAGGGACCAAGGTGGAGATCAGACGA11580.13986318047423038No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10860.1311670241753145No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10750.12983844474075792No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTACTACTCTGG10300.12440334705393549No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA9870.11920980926430517No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC9560.11546563085782752No Hit
GATCTATGGTGTATCTACTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTG9290.11220457224573406No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG9240.1116006725027538No Hit
CCATAGATCACGAGCCTAGGGGCTTGCCCTGGTTTCTGCTGATACCAATT9120.11015131311960115No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG9070.10954741337662087No Hit
ACTTACTACTGTCAACAGAGTTACACTATCCCTCTCACCTTCGGCAGAGG9070.10954741337662087No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG8690.1049577753299708No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8580.10362919589541422No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC8540.10314607610103001No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC8490.10254217635804973No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA8450.10205905656366551No Hit
GGATAGTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGC8440.10193827661506946No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8320.10048891723191682No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11400.051.8498761
GTATACG657.5467466E-444.3593671
TCAACGC12100.044.023184
ATCAACG12650.042.109133
TATCAAC13650.041.660982
GAATACG1252.874931E-740.3109559
CAACGCA14450.036.86375
GTACATG21250.036.29641
AACGCAG15250.034.9298676
CGCAGAG15200.034.097618
ACATGGG23350.032.986173
GTACACG4250.030.4872765
TACACGG4300.030.1327726
CATGGGG13700.029.9494844
ACACGGG4600.029.7324587
TTTCGCG1255.373117E-428.793549
ACGCAGA18300.028.7148677
GCAGAGT18950.027.3500639
GTATGTA7050.026.5841581
ACGGGGT5150.025.1594039
Produced by FastQC (version 0.11.5)