FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280089

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280089
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546408
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCTGTACTGTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGG18380.3363786767397256No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA17610.32228664294812664No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT16700.30563242119441886No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC16040.293553535087334No Hit
ACTACAACCCGTCCCTCATGAGTCGAGTCACCATATCAGCAGACACGTCC14240.26061111843164814No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13970.2556697559332953No Hit
CTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT13250.24249278927102091No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT12790.23407417168123454No Hit
GCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGGAGCAGGGCGCCAGGG11970.21906707076031098No Hit
CCTTCCCAGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG10420.19069998975124816No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG10420.19069998975124816No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT10230.18722273465981465No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG10190.18649068095635496No Hit
GTCCCTTACCTGTACTGTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACT9060.16581016383361882No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA8940.16361400272323975No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8820.1614178416128607No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGG8680.15885565365075183No Hit
CAATTATAGTGGGAGCACCTACTACAACCCGTCCCTCATGAGTCGAGTCA8610.15757455966969736No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC8430.15428031800412878No Hit
GGATTGGGAATATCAATTATAGTGGGAGCACCTACTACAACCCGTCCCTC8410.15391429115239894No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA8290.15171813004201987No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT8270.15135210319029002No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG8040.14714279439539685No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8000.14641074069193716No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAA7760.14201841847117905No Hit
GTAGTAGGTGCTCCCACTATAATTGATATTCCCAATCCACTCCAGCCCCT7650.14000527078666491No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAAC7650.14000527078666491No Hit
TCCTGGAGCAGGGCGCCAGGGGGAAGACCGATGGGCCCTTGGTGGAGGCT7490.13707705597282616No Hit
GTATCATAGTTATTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAC7420.1357959619917717No Hit
GTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCAG7410.1356129485659068No Hit
GTCCACCACCACGCACGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT7320.13396582773312252No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA7290.13341678745552774No Hit
ACCTACTACAACCCGTCCCTCATGAGTCGAGTCACCATATCAGCAGACAC7220.1321356934744733No Hit
GCTCCAGGAGCACCTCCGAGAGCACAGCGGCCCTGGGCTGCCTGGTCAAG7130.130488572641689No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7070.12939049208649947No Hit
GCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGTTCCACGACAC7030.12865843838303978No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGA6870.12573022356920105No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCTGGGAAGGTGTGCA6850.12536419671747118No Hit
CTGTTGAACTGCTCCTCCCGTGGCTTTGTCTTGGCATTATGCACCTCCAC6850.12536419671747118No Hit
GTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAAC6840.12518118329160627No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC6780.12408310273641675No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC6710.1228020087553623No Hit
CTATGATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCT6660.12188694162603769No Hit
GTGTAGGTCTGGGTGCCGAAGTTGCTGGAGGGCACGGTCACCACGCTGCT6550.11987379394152356No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCC6350.11621352542422513No Hit
GTCCTGAGGACTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGA6300.11529845829490051No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTCTTCCTCCT6290.11511544486903559No Hit
GTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGGCTCACGTCCA6250.1143833911655759No Hit
GTTCAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG6200.11346832403625129No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCTCTCGGAGGTGCTCCTGG6170.11291928375865654No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT6030.11035709579654764No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA5910.10816093468616857No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC5840.10687984070511412No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5830.10669682727924922No Hit
ATTATAGTGGGAGCACCTACTACAACCCGTCCCTCATGAGTCGAGTCACC5800.10614778700165443No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5790.10596477357578953No Hit
CGTCCACCACCACGCACGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG5780.1057817601499246No Hit
GACCAGCGGCGTGCACACCTTCCCAGCTGTCCTACAGTCCTCAGGACTCT5720.10468367959473508No Hit
GTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTGGAC5680.10395162589127538No Hit
GTATCAACGCAGAGTACATGGGGCTTTCTGAGAGTCATGGACCTCCTGTG5640.10321957218781569No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGG5590.10230450505849108No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)