FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences526128
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTGATACCACTGCTTGATACCACTGCTTGATACCACTGCTTGATACCA13630.25906243347626434No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT11840.22504029437703374No Hit
GTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAG10950.20812425873551685No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9110.17315178055530214No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7640.14521181157436971No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6980.13266733570537967No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6670.1267752334032783No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6640.12620502995468785No Hit
GAGTTACAGTACCCCTCCTACGTTCGGCCAAGGGACCAAGGTGGAAATCA6370.12107319891737371No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA6350.12069306328498007No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC6200.1178420460420278No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA5620.1068181127026123No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5580.10605784143782503No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA5350.1016862816652982No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGCG750.001532615938.407235
TACACGG3600.038.003546
GTACACG3550.036.5139165
ACACGGG4000.034.2031827
ACGGGGT4150.031.2318259
ATCAACG15000.029.28833
TCAACGC15000.029.2855154
GTATTAG3002.0008883E-1128.8191281
GAATACG2003.4494406E-728.8026819
AACGCAG15900.028.0780856
CAACGCA15650.028.069185
CGCAGAG16000.027.9025978
TAGGCGT1050.00796618627.4311266
GTATGTA5800.027.3284841
TATCAAC16700.026.740662
TTAGCAC1400.001038264425.7215753
GCAGCGC3151.1168595E-925.1451998
GTATATA5550.024.6650181
GCAGAGT18350.024.3292439
ACTATAC2401.6905524E-624.0068023
Produced by FastQC (version 0.11.5)