FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534785
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA15300.28609628168329326No Hit
GTTTATTACTGTGCGAATATAGGATATTGTACTGGTGGTGTGTGCTCTGA14490.27095000794711893No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT12580.23523472049515226No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10660.19933244200940567No Hit
GACCAGGGAAAGCCCCTAAGCTCCTGATCTACGATGCATCCAATTTGGAA10050.18792598894882992No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC9800.18325121310433165No Hit
ATATTATTGTCAGCAGTATGCCACTCTCCCTCCGTGGACGTTCGGCCAAG9110.17034883177351645No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG9100.17016184073973653No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA8220.15370662976710267No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8120.15183671942930335No Hit
CTATTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT8070.15090176426040372No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA7730.14454406911188608No Hit
CCGTAATAGTGATAGGGGGAGTGGTGGTCAGAGCACACACCACCAGTACA7590.14192619463896705No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7000.13089372364595117No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6990.13070673261217125No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC6910.1292108043419318No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC6880.128649831240592No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6740.12603195676767298No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC6650.12434903746365361No Hit
TCCCTGGTCTGTGCTGATACCAATTTAAATAGTTGTAAATGTCCTGACTC6260.11705638714623633No Hit
CAATAATATGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGTAT6250.1168693961124564No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6180.11556045887599689No Hit
GTACAATATCCTATATTCGCACAGTAATAAACAGCCGTGTCCTCAGCTCT6090.11387753957197752No Hit
ATATTGTACTGGTGGTGTGTGCTCTGACCACCACTCCCCCTATCACTATT6080.11369054853819759No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC6080.11369054853819759No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG5880.10995072786259899No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT5650.1056499340856606No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT5390.1007881672073824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC7950.045.2873424
ATCAACG8150.044.1759953
CAACGCA8300.043.3776325
TATCAAC9150.039.348022
CGCAGAG10100.034.9274988
GCAGCGC2700.034.6634948
GGCAGCG2456.002665E-1132.323577
GTATCAA12900.030.148051
GCAGAGT12050.029.2753289
GTATAGG1504.5984278E-528.8081381
CTGTGCG8950.028.1538459
CTATGCT2105.293805E-727.4260629
ACACTAT2405.6323188E-826.997536
GTACATG13350.026.4344331
CAGCGCT3006.348273E-1026.3975839
ACGAGTG1100.00999461626.1843195
TGGGGCG1400.001039426825.7167425
TATACAG2551.0115582E-725.414195
TACTGTG11550.024.9327817
TACATGG13600.024.3552672
Produced by FastQC (version 0.11.5)