FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456268
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA23790.5214040870716333No Hit
GTGTATTACTGTGCGAGAGGGTACGGTGACTACGTTTTTGTCCACTACTA15960.3497944190694942No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG11800.25861993389849824No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT10110.22158029929778114No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9920.21741608002314428No Hit
TAGTAGTAGTGGACAAAAACGTAGTCACCGTACCCTCTCGCACAGTAATA9580.20996431921589945No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC9400.2060192693767698No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA8910.1952799670369169No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC8870.1944032892948881No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCTCTCAGGCTG8480.1858556813101072No Hit
AATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGA8440.18497900356807842No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8050.1764313955832975No Hit
CTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCT8050.1764313955832975No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGT7700.16876046534054545No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG7660.16788378759851666No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG7560.16569209324344464No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC7220.15824033243619978No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGG7020.15385694372605574No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC6970.15276109654851971No Hit
GATGTACACTGGTACCAGCAGCTTCCGGGAACAGCCCCCAAACTCCTCAT6800.14903521614489731No Hit
CCGTAGTAGTAGTGGACAAAAACGTAGTCACCGTACCCTCTCGCACAGTA6690.14662435235431806No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCC6670.14618601348330368No Hit
GTACAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG6620.14509016630576765No Hit
GTATTTATTACTTCCATCATATGATATAACTGCCACCCACTCCAGCCCCT6620.14509016630576765No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACT6510.14267930251518846No Hit
ATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACAGCG6420.1407067775956236No Hit
CAGTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATA6140.13457003340142198No Hit
GTATTACTGTGCGAGAGGGTACGGTGACTACGTTTTTGTCCACTACTACT6040.13237833904634994No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA5750.1260224254166411No Hit
GGGCTGGAGTGGGTGGCAGTTATATCATATGATGGAAGTAATAAATACTA5590.12251571444852588No Hit
TGTGTGAGTTGTGTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCA5540.12141986727098986No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT5500.12054318952896105No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATGCTATGCACTGGG5480.12010485065794664No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC5330.11681730912533861No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCGGGAACAGCCCCCAAACT5320.1165981396898314No Hit
ATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATT5290.11594063138330982No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT5170.11331059815722339No Hit
TCCTATGACAGCAGCCTGAGAGGGGTGTTCGGCGGAGGGACCAAGCTGAC5040.11046139549562976No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGCATAGCTAC4970.10892720944707934No Hit
TGCCCACGGTGCCCAGAGCCCAAATCTTGTGACACACCTCCCCCGTGCCC4930.10805053170505055No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA4930.10805053170505055No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT4930.10805053170505055No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCTCTCAGGCTGC4870.10673551509200734No Hit
GTGCATAGCATAGCTACTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTC4860.10651634565650013No Hit
GTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC4830.10585883734997852No Hit
GTGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT4750.1041054818659209No Hit
GTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAGCTTCC4730.1036671429949065No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4600.10081794033331287No Hit
ATGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTG4600.10081794033331287No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG4580.10037960146229849No Hit
GTACGTGGACGGCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGG4570.10016043202679129No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)