FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences456268
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACA23150.5073772431991724No Hit
GTGTATTACTGTGCGAGAGGGTACGGTGACTACGTTTTTGTCCACTACTA15650.343000166568771No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG11470.2513873425267606No Hit
GTGTACACCTGTGGTTCTCGGGGCTGTCCTTTGGTTTTGGAGATGGTTTT11310.24788063155864537No Hit
TAGTAGTAGTGGACAAAAACGTAGTCACCGTACCCTCTCGCACAGTAATA9430.2066767776832914No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC9380.20558093050575538No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8600.18848571453619364No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC8380.1836639869550352No Hit
AATAAATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGA7900.17314385405068952No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC7870.1724863457441679No Hit
CTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCT7800.1709521596956175No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCTCTCAGGCTG7600.16656877098547346No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA7580.16613043211445905No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCCTGGAGCAGGGCGCCAGGG7390.1619662128398222No Hit
GTATTTATTACTTCCATCATATGATATAACTGCCACCCACTCCAGCCCCT7290.1597745184847502No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC7000.15341860485504133No Hit
GATGTACACTGGTACCAGCAGCTTCCGGGAACAGCCCCCAAACTCCTCAT6840.1499118938869261No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGGGTCTGGGGCCC6830.1496927244514189No Hit
CCCTTATGATTTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG6580.14421348856373886No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCC6500.14246013307968125No Hit
CCGTAGTAGTAGTGGACAAAAACGTAGTCACCGTACCCTCTCGCACAGTA6230.1365425583209868No Hit
CAGTAATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATA6200.1358850500144652No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAAGTGGTACGT6190.13566588057895798No Hit
ATACACAGCCGTGTCCTCAGCTCTCAGGCTGTTCATTTGCAGATACAGCG6170.13522754170794357No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCAGGTTATGATGTACACT6050.13259750848185714No Hit
TCATAAGGGTATCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT6050.13259750848185714No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC6000.13150166130432114No Hit
GTATTACTGTGCGAGAGGGTACGGTGACTACGTTTTTGTCCACTACTACT5990.13128249186881394No Hit
GTGTTGTAGTTGTTCTCCGGCTGCCCGCTGCTCTCCCACTCCACGGCGAT5900.12930996694924912No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC5700.1249265782391051No Hit
GTACAACAGCACGTTCCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG5690.12470740880359789No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGT5670.12426906993258348No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCGGGAACAGCCCCCAAACT5590.12251571444852588No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA5480.12010485065794664No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTAGCTATGCTATGCACTGGG5440.11922817291591785No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG5420.11878983404490344No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGCATAGCTAC5240.11484478420577379No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT5220.11440644533475941No Hit
TCCTATGACAGCAGCCTGAGAGGGGTGTTCGGCGGAGGGACCAAGCTGAC5190.1137489370282378No Hit
GTGCATAGCATAGCTACTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTC4950.10848887057606493No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGAACGTGCTGTTGTACTGCT4930.10805053170505055No Hit
GTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC4930.10805053170505055No Hit
GGGCTGGAGTGGGTGGCAGTTATATCATATGATGGAAGTAATAAATACTA4910.10761219283403614No Hit
GCTGTGTATTACTGTGCGAGAGGGTACGGTGACTACGTTTTTGTCCACTA4900.10739302339852894No Hit
ATACTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATT4860.10651634565650013No Hit
TGTGTGAGTTGTGTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCA4820.10563966791447131No Hit
GTGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT4770.10454382073693531No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA4750.1041054818659209No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT4750.1041054818659209No Hit
CTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT4670.10235212638186328No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAT400.006205113654.0253831
GGTATCA3850.052.3882521
GTATCAA7050.050.066081
GTATAGG1202.0726657E-742.0197451
ATCAACG8050.041.1621973
TCAACGC8150.040.652684
AACGCAG8200.040.387086
TATCAAC8250.040.164332
CAACGCA8250.040.1599245
CTAACAC1001.4440215E-436.0169223
CGCAGAG9200.035.9971778
TACACGG3800.032.2086
GTACACG4100.031.6211475
TATGTAC4350.031.4630582
CTACACA3900.031.3959224
GCAGAGT10550.031.39099
GTATTAG950.004876055330.3300421
ACGGGGT4100.029.851329
TATAGAG1000.006265388328.813542
ACACGGG4250.028.7977397
Produced by FastQC (version 0.11.5)