FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109792
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTACACTGGTCATCTATCAAGATATCGAGCGGCCCTCAGGGATCCCT81880.7377959113059024No Hit
CTATAGTACAATAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATT52960.4772065396038177No Hit
GTACTATAGAGTCCCAATGTAGTAGTACCAGCTGCTACGGGTCTTCTTGG52580.4737824745537903No Hit
GGATATGTGGCCTCCTATGAACTGACTCAGCCACCCTCAGTGTCCGTGTC29140.2625717251521006No Hit
CCTATGAACTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA28480.256624664802053No Hit
CCCTTAGAGTCTCCTGTGCAGCCTCTGGATTCACTCTCACCAACGCCTGG26370.2376120930769009No Hit
GTGTAGGGGTCTGGCCTGGCTTCTGCTGATACCAACAAGAATATTTATTT24400.21986101900175892No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC21420.19300914045154408No Hit
GTATTATTGTACTATAGAGTCCCAATGTAGTAGTACCAGCTGCTACGGGT19290.1738163547763905No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT18070.16282330382630258No Hit
TTATTGTACTATAGAGTCCCAATGTAGTAGTACCAGCTGCTACGGGTCTT18060.16273319685130186No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17640.15894870390127158No Hit
ATAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGAGACA17270.1556147458262449No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG17260.1555246388512442No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC17030.15345217842622763No Hit
CAATAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGAGA16120.14525244370116203No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC15890.14317998327614545No Hit
GTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGC15850.14281955537614255No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA15720.1416481647011332No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15640.14092730890112742No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT15310.13795377872610362No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA15250.13741313687609932No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAG14990.13507035552608057No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14950.1347099276260777No Hit
ATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGAGACAATG14800.13335832300106687No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14660.1320968253510568No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA14560.13119575560104957No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG14490.13056500677604452No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG14090.1269607277760157No Hit
GACTATTACTGTCAGGTGTGGGACAGCAGCACTGTGCTATTCGGCGGAGG14070.12678051382601424No Hit
GAATAGCACAGTGCTGCTGTCCCACACCTGACAGTAATAGTCAGCCTCAT14030.12642008592601137No Hit
GTACAATAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAG14020.12632997895101064No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13870.12497837432599983No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC13820.12452783945099623No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT13730.12371687667598974No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCCAGCCTTGGGATTCCCAAGTGT13610.12263559297598108No Hit
GCCGTGTATTATTGTACTATAGAGTCCCAATGTAGTAGTACCAGCTGCTA13570.1222751650759782No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13410.12083345347596666No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA13410.12083345347596666No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13390.12065323952596523No Hit
GTGCTGCTGTCCCACACCTGACAGTAATAGTCAGCCTCATCGATGGCCTG13360.12038291860096306No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13340.12020270465096163No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13260.11948184885095586No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13210.11903131397595225No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13140.11840056515094721No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13060.11767970935094145No Hit
GTGTATTATTGTACTATAGAGTCCCAATGTAGTAGTACCAGCTGCTACGG13020.11731928145093855No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA12980.11695885355093567No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12940.11659842565093279No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA12780.11515671405092126No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGATAAAGCCTGGGG12410.11182275597589458No Hit
GTCCCAATGTAGTAGTACCAGCTGCTACGGGTCTTCTTGGTTCGACCCCT12390.11164254202589315No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACAGTGCTGCTG12370.1114623280758917No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT12280.11065136530088521No Hit
GTAATAGTCAGCCTCATCGATGGCCTGGGCCCCGCTGATGGTCAGAGTGG12210.11002061647588017No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA12050.10857890487586863No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC12010.10821847697586576No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG12000.10812837000086503No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT11990.10803826302586429No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA11510.1037131282258297No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT11350.10227141662581818No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11170.1006494910758052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24250.060.686261
ATCAACG25000.057.6385653
TACCGTT502.0831442E-457.5840347
TCAACGC25700.056.068644
TATCAAC26000.055.4216962
CAACGCA26200.054.9961555
AACGCAG27050.053.2223936
ACGCAGA30150.047.5092167
CGCAGAG30300.047.274028
GCAGAGT35000.041.337119
GAATACG4050.040.8775529
TATAGAG28150.038.9017225
CTATAGA27900.038.7355964
GTACTAT27700.038.5437431
TACTATA28650.038.224532
ACTATAG27700.037.974873
TCTACAC9200.036.807243
TACACTG37100.036.507965
ATAGAGT29000.036.488086
CCTACAC35150.036.2803233
Produced by FastQC (version 0.11.5)