FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109792
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTACACTGGTCATCTATCAAGATATCGAGCGGCCCTCAGGGATCCCT70410.6344432109800756No Hit
GTACTATAGAGTCCCAATGTAGTAGTACCAGCTGCTACGGGTCTTCTTGG48900.440623107753525No Hit
CTATAGTACAATAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATT46680.42061935930336497No Hit
GTGTAGGGGTCTGGCCTGGCTTCTGCTGATACCAACAAGAATATTTATTT27720.2497765347019982No Hit
GGATATGTGGCCTCCTATGAACTGACTCAGCCACCCTCAGTGTCCGTGTC26430.2381527349269052No Hit
CCCTTAGAGTCTCCTGTGCAGCCTCTGGATTCACTCTCACCAACGCCTGG24650.22211369337677692No Hit
CCTATGAACTGACTCAGCCACCCTCAGTGTCCGTGTCCCCAGGACAGACA23580.21247224705169976No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC19800.1784118105014273No Hit
GTATTATTGTACTATAGAGTCCCAATGTAGTAGTACCAGCTGCTACGGGT18650.16804950837634441No Hit
GTGCTATTCGGCGGAGGGACCAAGCTGACCGTCCTACGTCAGCCCAAGGC17200.15498399700123985No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17060.15372249935122978No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT16750.15092918312620746No Hit
TTATTGTACTATAGAGTCCCAATGTAGTAGTACCAGCTGCTACGGGTCTT16380.14759522505118078No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15800.14236902050113895No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC15230.13723292292609787No Hit
CAATAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGAGA15220.13714281595109712No Hit
ATAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGAGACA15120.13624174620108992No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA15080.13588131830108705No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA14790.13326821602606614No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA14720.1326374672010611No Hit
GTACAATAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAG14140.1274112626510193No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCT13960.12578933710100632No Hit
GTGCTGCTGTCCCACACCTGACAGTAATAGTCAGCCTCATCGATGGCCTG13840.12470805340099766No Hit
GAATAGCACAGTGCTGCTGTCCCACACCTGACAGTAATAGTCAGCCTCAT13820.12452783945099623No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACAG13610.12263559297598108No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13540.12200484415097604No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG13470.12137409532597099No Hit
ATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGAGACAATG13430.12101366742596811No Hit
GTGTATTATTGTACTATAGAGTCCCAATGTAGTAGTACCAGCTGCTACGG13400.12074334650096594No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13300.11984227675095874No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA13270.11957195582595657No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13220.11912142095095296No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13130.11831045817594647No Hit
GACTATTACTGTCAGGTGTGGGACAGCAGCACTGTGCTATTCGGCGGAGG13000.11713906750093711No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC12970.11686874657593496No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12850.11578746287592628No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCCAGCCTTGGGATTCCCAAGTGT12830.11560724892592485No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12820.11551714195092413No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12620.11371500245090972No Hit
GCCGTGTATTATTGTACTATAGAGTCCCAATGTAGTAGTACCAGCTGCTA12610.113624895475909No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12420.11191286295089531No Hit
GTAATAGTCAGCCTCATCGATGGCCTGGGCCCCGCTGATGGTCAGAGTGG12390.11164254202589315No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA12370.1114623280758917No Hit
GTCCCAATGTAGTAGTACCAGCTGCTACGGGTCTTCTTGGTTCGACCCCT12340.11119200715088953No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA12340.11119200715088953No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12310.11092168622588738No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11960.10776794210086214No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGATAAAGCCTGGGG11960.10776794210086214No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACAGTGCTGCTG11880.10704708630085637No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11640.10488451890083908No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG11470.10335270032582683No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA11360.1023615236008189No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT11150.10046927712580377No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC11120.10019895620080159No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA11120.10019895620080159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC17350.058.844344
ATCAACG17650.057.8415453
GTATCAA18150.057.1150631
CAACGCA19150.052.9426165
TATCAAC19550.052.2201162
AACGCAG19450.052.1330766
CGCAGAG21650.046.83768
ACGCAGA21600.046.6109667
GCAGAGT25550.039.688229
CTATAGT22550.037.3511621
GTACATG30850.037.1028021
GAATACG3900.036.880679
TACATGG31150.036.4666022
GTACTAT27450.036.453331
TATAGAG27400.036.214595
ATAGTAC23700.036.0989723
ACTATAG27100.036.079953
TACTATA28000.035.94732
TATAGTA24850.035.296342
CTATAGA28750.035.0111244
Produced by FastQC (version 0.11.5)