FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268843
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT29820.2350172558779928No Hit
ACCATATACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGGGA25220.19876375564195098No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22150.1745684848322448No Hit
GTAGTAGACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGAAGCA20990.1654262978161995No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT20460.1612492640933512No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC18350.14461994115899288No Hit
GTATATGGTATTACTACTACCACTAATGTATGAAACCCACTCCAGCCCCT18230.1436741976745744No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16510.13011854106457615No Hit
TACTTAGCCTGGTACCAACACAAGCCTGGCCAGGCTCCCAGGCTCCTCAT15320.12073991817742621No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14730.11609001271236867No Hit
GTCTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC14430.11372565400132245No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGGAGATT14050.11073079963399726No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA13860.10923337245033467No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13830.10899693657923007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG18650.059.3665473
TCAACGC19000.059.0320784
GTATCAA20800.055.3564341
CAACGCA20550.054.2339485
AACGCAG21300.051.9949346
TATCAAC23600.048.1332322
CGCAGAG25250.043.861078
GGTATCA9050.041.3491171
GCAGAGT31750.035.1081479
GTACATG36400.034.0046651
GAATACG3900.033.191579
TACATGG37100.032.1687472
TAGACGG10700.031.5838785
ACATGGG36850.031.4114743
ACGCAGA36150.030.8349537
GTAGACG11500.030.6348194
GTGCTAG5750.030.0368821
TATACCG1703.2127664E-629.6074145
ACTACGC13850.028.5583328
AGACGGC12450.027.7262946
Produced by FastQC (version 0.11.5)