FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408734
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT38330.27208827216493675No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33240.23595653970160443No Hit
ATATTATAATCTCCCTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGC32280.2291419103961429No Hit
TTATAATATTGTTGACAGTAATATGTTGCAATATCTTCAGTCTGCAGGCT28510.20238029322782017No Hit
ATTATAATCTCCCTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGCTC26820.19038370622133063No Hit
GTAATACACAGCCGTGTCTTCAGATCTCAGGCTGTTCAGCTCCATGTAGG26630.18903497750462472No Hit
ATATTACTGTCAACAATATTATAATCTCCCTCCTCTCACTTTCGGCGGAG26060.18498879135450697No Hit
ACATATTACTGTCAACAATATTATAATCTCCCTCCTCTCACTTTCGGCGG25560.18143950525791244No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAA24470.17370206156733636No Hit
GTGTATTACTGTGCGACTAACCGCCCTGGTCGTATAGCACCTCACCATTA22300.15829815990811608No Hit
GTAATATGTTGCAATATCTTCAGTCTGCAGGCTGCTGATGGTGAAAGTAA21950.1558136596404999No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC21940.155742673918568No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG20290.1440300297998061No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG19260.13671850044082134No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18300.12990387113535984No Hit
CAGTAATATGTTGCAATATCTTCAGTCTGCAGGCTGCTGATGGTGAAAGT17500.12422501338080857No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG17360.12323121327376212No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT17020.12081769872807785No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT16710.11861714134818924No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16640.11812024129466599No Hit
GATTATAATATTGTTGACAGTAATATGTTGCAATATCTTCAGTCTGCAGG16200.11499686952966282No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16100.1142870123103439No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15950.11322222648136554No Hit
GCTATACATTGGGTGCGCCAGGCCCCCGGACAAAGCCTTGAGTGGCTGGG15840.11244138354011475No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15580.11059575476988558No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15360.10903406888738398No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGTA15220.10804026878033753No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14670.10413605407408354No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14260.10122563947487602No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24000.074.241551
ATCAACG28500.060.4061663
TCAACGC28500.060.4061664
CAACGCA29750.057.8639765
TATCAAC31350.055.1444632
AACGCAG31800.054.0895586
CGCAGAG36000.047.775728
ACGCAGA39400.043.6529437
GCAGAGT41800.041.318719
GTACATG39850.031.4979461
ACATGGG39300.031.3423633
CAGCGCT7600.029.3534989
CAGAGTA41200.027.4229559
CATGGGG24300.027.2715474
GTATATA6850.025.2744661
GTACTAA3151.0968506E-925.1908841
CTGTGCG17950.024.0546289
GCAGCGC9600.023.9878068
CCTATAC4303.6379788E-1223.4524273
TACACGG9550.023.3615136
Produced by FastQC (version 0.11.5)