FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1408734
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT36630.26002069943651535No Hit
ATATTATAATCTCCCTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGC32000.22715431018204998No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC29430.20891097964555408No Hit
TTATAATATTGTTGACAGTAATATGTTGCAATATCTTCAGTCTGCAGGCT25880.18371104835973293No Hit
ATATTACTGTCAACAATATTATAATCTCCCTCCTCTCACTTTCGGCGGAG25040.17774824771745412No Hit
GTAATACACAGCCGTGTCTTCAGATCTCAGGCTGTTCAGCTCCATGTAGG24700.17533473317176984No Hit
ATTATAATCTCCCTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGCTC24680.17519276172790604No Hit
ACATATTACTGTCAACAATATTATAATCTCCCTCCTCTCACTTTCGGCGG22600.1604277315660728No Hit
GTGTATTACTGTGCGACTAACCGCCCTGGTCGTATAGCACCTCACCATTA21200.1504897304956081No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTAGCAA21150.15013480188594866No Hit
GTAATATGTTGCAATATCTTCAGTCTGCAGGCTGCTGATGGTGAAAGTAA20380.14466890129719306No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19750.140196800815484No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG18580.13189147134945278No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18540.13160752846172522No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT16210.11506785525159469No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG16150.11464194092000335No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT16050.11393208370068443No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGATTTCCTGTA15980.11343518364716121No Hit
CAGTAATATGTTGCAATATCTTCAGTCTGCAGGCTGCTGATGGTGAAAGT15530.11024082616022614No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG15190.10782731161454184No Hit
GATTATAATATTGTTGACAGTAATATGTTGCAATATCTTCAGTCTGCAGG14750.10470393984953867No Hit
GCTATACATTGGGTGCGCCAGGCCCCCGGACAAAGCCTTGAGTGGCTGGG14720.104490982683743No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14720.104490982683743No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14260.10122563947487602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)