FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781362
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC23070.29525367243351996No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAAATTGTTGTTG15800.20221101102945882No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15040.19248440543563675No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13630.1744389924260458No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA13600.17405504746839492No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAA12880.164840368484774No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12770.16343257030672081No Hit
CTCCTGGACAGTCAGTCGCCATCTCCTGCACTGGAACCAGCAGTGACATT12120.15511376289095194No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC12040.15408990967054964No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGGTTATAACTTTGTCTCCT12000.15357798306034848No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11880.152042203229745No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATCACTGCAGCTCATCT11560.14794679034813568No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11350.1452591756445796No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT11320.1448752306869287No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA11300.14461926738182812No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTC11210.14346743250887553No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10400.133100918652302No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10380.13284495534720142No Hit
GTGATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGG10090.12913348742324302No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9840.12593394610948574No Hit
GTAATACACGGCTGTGTCCTCGGTTTTCAGGCTGTTCATTTGCAGATACA9770.1250380745416337No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG9760.12491009288908342No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA9690.1240142213212314No Hit
CTCCTGTACAGCCTCTGGATTCACTTTCAGTGACGCCTGGATGAGCTGGG9600.12286238644827878No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA9470.121198624965125No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAAATTGTTGTTGC9250.11838302860901861No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA9180.11748715704116656No Hit
CCCTTAGACTCTCCTGTACAGCCTCTGGATTCACTTTCAGTGACGCCTGG9180.11748715704116656No Hit
GATTATCACTGCAGCTCATCTGCAGGCAACAACAATTTGGTATTCGGCGG9110.11659128547331454No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8860.1133917441595573No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC8800.11262385424425554No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG8700.11134403771875265No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG8680.11108807441365205No Hit
GCTTGATACCACTGCTTGATACCACTGCTTGATACCACTGCTTGATACCA8500.10878440466774682No Hit
TCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTG8460.10827247805754567No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT8450.10814449640499538No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8430.1078885330998948No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTTTGATGTC8310.1063527532692913No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG8260.10571284500653987No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT8200.10494495509123813No Hit
GGTTATAACTTTGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAA8150.10430504682848667No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA1854.465619E-935.0316351
CGGTACA850.002835288433.8846444
AAGCGTC850.002836178333.8824735
ATCAACG39850.029.6331183
TCAACGC39950.029.5589434
CAACGCA41150.028.870095
CTATACT3750.028.8019474
AACGCAG41400.028.6957516
TATCAAC42100.028.3914682
GTATATA4700.027.5780961
CGCAGAG42900.027.3549928
ACGCAGA43150.027.1965037
ACACCGT1607.1553404E-527.0000956
ATTAGAC2757.2723196E-926.1835883
TTAGACT9450.025.9065134
TACACGG6400.025.8750925
GTATCAA50450.025.5494461
CCTAGGT15900.024.0016212
ATTGAGT6950.023.8258977
ATACACG6650.023.821164
Produced by FastQC (version 0.11.5)