FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280105

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280105
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611405
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12470.20395646093833059No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10720.17533386216992008No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7710.1261029922882541No Hit
GCTTGATACCACTGCTTGATACCACTGCTTGATACCACTGCTTGATACCA7460.1220140496070526No Hit
GTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAG7440.1216869341925565No Hit
GGAATACACAGCCGTATCTTCAACTTTCAGGCTATTCATTTGCAGAGACA6610.10811164449096754No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGC1051.9259365E-434.2973251
CCTATAC2456.002665E-1132.3321883
GTAGCAC1402.8743854E-530.8625413
CTAGTAG2603.9908628E-927.6971513
CTATACC2955.2023097E-1026.8521544
CCTAGTA2156.489281E-726.7953852
GTATAAC1907.5244807E-626.5352951
CCCTAGT1701.07779044E-425.420371
TCAACGC30550.021.9224
ATCAACG30700.021.814893
CAACGCA31150.021.497995
GGAGTTA6700.021.4910267
GTATAGG2353.8991253E-521.454071
AACGCAG31650.021.3858786
CGCAGAG32400.021.1096238
ACTGACC7900.020.960558
CTATACT3106.653481E-720.9068854
AGTTACC6900.020.8680959
TTAGGGC1750.003802874820.5750273
CTAACAC8750.020.5750273
Produced by FastQC (version 0.11.5)