FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences611405
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12480.2041200186455786No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10360.16944578470898994No Hit
GCTTGATACCACTGCTTGATACCACTGCTTGATACCACTGCTTGATACCA8570.14016895511158722No Hit
GTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAG6700.10958366385620005No Hit
GGAATACACAGCCGTATCTTCAACTTTCAGGCTATTCATTTGCAGAGACA6180.10107866307930094No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA1905.7589205E-934.1285321
AATCCGC1050.0079761127.4248316
CCGCTAC1400.001041004725.7107779
GACTCGG3201.3442332E-924.7466267
CTTAGTC2351.4062261E-624.5213383
GTATAGA2701.7489765E-724.0163761
CCTATAC1500.001551078924.0104773
GTATTAT5200.023.5545221
GTCTAAT2802.4846668E-723.1586481
GTACTAT2852.9471812E-722.7523561
CATGGGG9750.021.4247324
ATCCGCT1700.003220573521.1735847
CTTAGGC2404.582663E-521.0091693
GTACAGA4201.36788E-920.5854631
CTAGGAC2455.3714168E-520.580413
GTTATAC1750.00379711620.5804083
CTTACAC3951.5183105E-820.0593853
TATACAG4001.7544153E-819.8086435
TATAGAG2557.307131E-519.7733352
GAGGTCT4803.43789E-1019.4973419
Produced by FastQC (version 0.11.5)