FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1253238
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC52540.4192340162044241No Hit
GTGTGGAGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41730.3329774552000498No Hit
CTCCTGCACTGCAACCAGCAGTGACGTTGGTGATTATAACTATGTCTCCT41670.33249869537948895No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTTTC35220.2810320146692009No Hit
GTTTGGAGGGTGTGGAGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT34170.2726537178093866No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACAACATAACTGTTG33280.2655521138044011No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC31100.24815717365735795No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT30380.24241205581062814No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT29850.23818301072900758No Hit
ATAATAATCAGCCCCATCCCCAGCCTGGAGCCCAGAGACGGTCAGGTAGG29460.23507107189536225No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA28820.22996430047604685No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA28600.22820884780065717No Hit
GATTATTATTGCAGCTCATATGCAGGCAGCAACAGTTATGTTGTATTCGG27490.219351791120282No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG27050.21584088576950267No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26790.2137662598804058No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26500.21145225408102852No Hit
GATTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAA26190.20897866167479762No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA26110.2083403152473832No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACAAC26050.20786155542682236No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT25580.2041112701657626No Hit
TCTCTGGGCTCCAGGCTGGGGATGGGGCTGATTATTATTGCAGCTCATAT25190.20099933133211728No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA24850.19828635901560598No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG24320.1940573139339854No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA23920.19086558179691326No Hit
TTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGCCCCCAAAC23660.1887909559078164No Hit
TACTGGAACTATGCGAAAGACTTTGACCACTGGGGCCAGGGAACCCTGGT23210.18520025725361025No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA22810.18200852511653812No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT22740.1814499719925505No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGAC22430.1789763795863196No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22380.17857741306918556No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21000.16756593719628674No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT20810.16604986443117747No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC20680.16501255148662902No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT20210.16126226622556927No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG19500.15559694168226626No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG19480.15543735507541265No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA19110.15248500284862093No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT18580.14825595776700037No Hit
TTTCTGTACCACGGCGGGGGGAGATTACTGGAACTATGCGAAAGACTTTG18120.1445854658093674No Hit
GGTACCAACAGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGACGTC18010.1437077394716726No Hit
TCACTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCT17820.14219166670656333No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT17680.14107456045858807No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC17180.1370848952872479No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17160.13692530868039432No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT16950.13524964930843145No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAATACAACATAACTGTTGC16820.134212336363883No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC16560.13213771047478612No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGT16420.13102060422681086No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG16400.13086101761995728No Hit
CCTCCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16390.13078122431653044No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16320.13022267119254283No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGG16300.13006308458568921No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16250.1296641180685552No Hit
GACCTACACCTGCAACGTAGATCACAAGCCCAGCAACACCAAGGTGGACA16130.1287065984274336No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG16130.1287065984274336No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACGTGCGTGGTGGTGGACGTG15750.12567445289721504No Hit
GTTATGTTGTATTCGGCGGAGGGACCAAACTGACCGTCCTAGGTCAGCCC15630.12471693325609341No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG15390.12280189397385013No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC15290.12200396093958212No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG15250.1216847877258749No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC15090.12040809487104603No Hit
GCCCTGACTCAGCCTCCCTCCGCGTCCGGGTTTCCTGGTCAGTCAGTCAC15040.12000912835391203No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14870.11865264219565637No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA14860.11857284889222958No Hit
ACATAACTGTTGCTGCCTGCATATGAGCTGCAATAATAATCAGCCCCATC14600.11649822300313269No Hit
GTCAGTCACCATCTCCTGCACTGCAACCAGCAGTGACGTTGGTGATTATA14190.11322669756263376No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG14130.11274793774207292No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGTAACACGG13830.11035413863926885No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG13500.10772095962618433No Hit
CTCCTGTGCAGCCTCCGGATTCAGTTTCAATGACGCCTGGATGAGCTGGG13380.1067634399850627No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCT13310.10620488686107507No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA13230.10556654043366064No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT13040.10405046766855139No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGGCCTCCCAGCCCCCATCGAGAA13010.10381108775827097No Hit
CATATGCAGGCAGCAACAGTTATGTTGTATTCGGCGGAGGGACCAAACTG13000.10373129445484418No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGACCTCCAGCAAC12890.10285356811714935No Hit
GCTCATATGCAGGCAGCAACAGTTATGTTGTATTCGGCGGAGGGACCAAA12820.10229501499316171No Hit
GTTCCAGTAATCTCCCCCCGCCGTGGTACAGAAATACACGCCTGTGTCCG12780.1019758417794545No Hit
CATATGAGCTGCAATAATAATCAGCCCCATCCCCAGCCTGGAGCCCAGAG12770.1018960484760277No Hit
CCCTCAGACTCTCCTGTGCAGCCTCCGGATTCAGTTTCAATGACGCCTGG12620.10069914892462564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22700.081.023551
GGTATCA9000.064.112761
TATCAAC28650.063.8555562
ATCAACG28450.063.798123
TCAACGC28600.063.211684
CAACGCA29900.060.4609265
AACGCAG30750.058.986196
ACGCAGA34100.053.187127
CGCAGAG35050.051.745528
TTATACG752.6517984E-548.0173654
GCAGAGT38250.047.416499
TATTGAG21200.037.7102475
TTATTGA22350.035.7713284
ATTGAGG10500.033.5893556
GTACATG33850.032.387831
GTACACG12600.031.4386885
ACATGGG33500.031.1754553
TACATGG34350.031.0330582
TACACGG12850.030.8073626
GTATGTA14800.029.7279591
Produced by FastQC (version 0.11.5)