FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076688
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT30620.28439064984470896No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24330.2259707547590388No Hit
GCATAAAGCAGGGAAAGCCCCTAATCTCCTGATCTATGCTGCAGTCAGTT20170.18733374942416003No Hit
GAGTAGATATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG19180.17813888517379223No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCGTTAGCAG18690.1735878917569435No Hit
TATCTACTCTGGTCCCACCGCCGAAAGTGAGCGGGAGACTGTCACTCTGT18180.16885114350675406No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16070.1492540085893035No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15660.14544603450581786No Hit
GCATGGACATGAGGGTCTCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG15430.14330985392239906No Hit
GTCTACAGAGTGACAGTCTCCCGCTCACTTTCGGCGGTGGGACCAGAGTA15020.1395018798389134No Hit
CCGTAATAGTCACTGGATAGTTCCCGCACTCTCGCACAAAAATAGACGGC14920.1385731056722096No Hit
CTCCTGATCTATGCTGCAGTCAGTTTGCAAAGTGGTGTCCCACCAAGGTT14890.13829447342219844No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14420.13392923483869051No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14350.13327909292199783No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC13670.12696342858841186No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13560.12594177700503767No Hit
CTATTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT13190.12250531258823354No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG12780.1186973385047479No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12750.11841870625473674No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA12730.11823295142139598No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12600.11702554500468101No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT12390.11507511925460301No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12140.11275318383784345No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT11940.11089563550443582No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11710.10875945492101705No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG11660.10829506783766514No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC11360.1055087453375537No Hit
ACTGTCTACAGAGTGACAGTCTCCCGCTCACTTTCGGCGGTGGGACCAGA11340.10532299050421293No Hit
GTCTCTGGTGGCTCCCTTAGCAGAAGCACTTACTACTGTGCCTGGATCCG11270.10467284858752025No Hit
CACTTACTACTGTGCCTGGATCCGCCAGACTCCACGGAAGGGTCTGGAGT11220.10420846150416833No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11190.10392982925415718No Hit
GTAGTAAGTGGCAAAATCTTCAGCTTGGACACTGCTGATGGTGAGAGTGA11160.10365119700414605No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11050.10262954542077185No Hit
ACTTACTACTGTCTACAGAGTGACAGTCTCCCGCTCACTTTCGGCGGTGG11020.1023509131707607No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11000.10216515833741993No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11000.10216515833741993No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA10940.10160789383739766No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10910.1013292615873865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21500.077.181141
TCAACGC24850.066.405724
ATCAACG25000.066.0072863
TATCAAC26600.062.3078272
ACGCAGA29850.055.21067
CGCAGAG30150.054.8999378
CAACGCA31950.052.325525
AACGCAG33100.050.446576
GCAGAGT35700.046.566669
TATACCG700.001088772941.177355
GAATACG3700.038.9008949
GTGCTAG3900.038.8486981
GTACATG29250.033.0522271
TACATGG28650.032.9490622
CATGGGG19350.028.6751824
CTAGAGA6000.027.6231384
GCTAGAG6350.024.9657963
ATGGGGC8900.023.4803415
CTATATA3402.6502676E-923.3418671
AGAATAC6800.023.2833298
Produced by FastQC (version 0.11.5)