FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1076688
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT28520.26488639234392874No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21570.20033658775801344No Hit
GAGTAGATATCAGACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG17990.1670864725900168No Hit
TATCTACTCTGGTCCCACCGCCGAAAGTGAGCGGGAGACTGTCACTCTGT17700.16439302750657572No Hit
GCATAAAGCAGGGAAAGCCCCTAATCTCCTGATCTATGCTGCAGTCAGTT17300.16067793083976045No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCGTTAGCAG15220.14135942817232106No Hit
CTCCTGATCTATGCTGCAGTCAGTTTGCAAAGTGGTGTCCCACCAAGGTT15090.1401520217556061No Hit
GTCTACAGAGTGACAGTCTCCCGCTCACTTTCGGCGGTGGGACCAGAGTA14870.1381087185888577No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14460.13430074450537202No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13990.1299355059218641No Hit
CCGTAATAGTCACTGGATAGTTCCCGCACTCTCGCACAAAAATAGACGGC13530.12566314475502655No Hit
GCATGGACATGAGGGTCTCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13320.1237127190049485No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13290.12343408675493737No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12930.12009049975480361No Hit
CTATTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCT12230.1135890805878769No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT12040.11182440967113964No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA11840.10996686133773201No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11640.10810931300432437No Hit
GTAGTAAGTGGCAAAATCTTCAGCTTGGACACTGCTGATGGTGAGAGTGA11550.10727341625429093No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT11460.10643751950425749No Hit
GTCCGTAGACACGTCGAGGAACCAGTTCTCCCTGAACCTGAGGTCTGTGA11190.10392982925415718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG13200.055.539913
TCAACGC13850.053.4572684
GTATCAA14400.052.4748761
GAATACG3800.049.2120829
TATCAAC16350.045.720892
ACGCAGA17750.042.1421787
ATAACCG700.001101381441.0810785
GTGCTAG4600.040.6761781
CGCAGAG19300.038.3850338
CAACGCA21200.035.2677235
GTACATG27900.035.079981
GCTAGAG6050.034.4525643
AACGCAG22300.033.5436636
CTAGAGA6450.033.4334224
TACATGG28350.033.2137072
GCAGAGT22700.032.9525839
TCTACAC9600.029.1992913
TGCTAGA8750.026.2870032
ACATGGG35850.025.0610793
AGAATAC8650.024.945218
Produced by FastQC (version 0.11.5)