FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280113

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280113
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences832617
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT33550.4029463727019746No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC30300.36391281945960746No Hit
GTATGATAATCTCCCTCTCACTTTCGGCGGGGGGACCAGGGTGGAGATCA26680.32043544630964776No Hit
TCACTAGACAGGTCCAAAAACCAGATCTCCCTGAAGATGACCTCTGTGAC23520.28248282223399235No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAAGCGAGTCAGGGCATTAGCAA20370.24465030139908264No Hit
TTATCATACTGCTGACAGAAATAGGTTGCAATATCCTCAGGCTGCAGGCT19730.23696369399135497No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17420.20921984537908786No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG17100.20537654167522404No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16710.20069251528614No Hit
CATTTTACTTTCACCATCAGCAGCCTGCAGCCTGAGGATATTGCAACCTA14460.1736692861183473No Hit
GTAATACACGGCCGTGTCCGCGGCGGTCACAGAGGTCATCTTCAGGGAGA14240.17102701482194094No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14020.16838474352553454No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13470.16177906528451858No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT13200.15853627778438345No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13180.15829607130289194No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT12790.15361204491380792No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12210.14664605695055472No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11990.14400378565414831No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11520.1383589333390983No Hit
ACTATAACCCGTCCCTCAAGAGTCGAGTCTCCATTTCACTAGACAGGTCC11400.13691769445014934No Hit
GTCTCTGGTGGCTCCATCAACGGTGGTGATTACTCCTGGAACTGGATCCG11230.1348759393574717No Hit
GTCCCAACCAGGAGACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG10840.1301919129683876No Hit
ATTTAAATTGGTATCAACTGAAACCAGGGAAAGCCCCTAAGCTCCTGATC10740.12899088056093017No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10630.12766974491272698No Hit
GTGTATTACTGTGCCAAAGCGGGGAGACTGGGGTCGGGTTCATACGGTTT10590.127189331949744No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA10420.12514757685706634No Hit
GTCTAGTGAAATGGAGACTCGACTCTTGAGGGACGGGTTATAGTCGGTAG10250.12310582176438868No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA10140.12178468611618548No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10120.12154447963469397No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA9820.11794138241232163No Hit
ATGATAATCTCCCTCTCACTTTCGGCGGGGGGACCAGGGTGGAGATCAAA9780.11746096944933865No Hit
GATCTACGATGCATCCATTTTGGAGACGGGGGTCCCATCAAGGTTCAGTG9710.11662024676411843No Hit
GGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG9520.11433828518994929No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9450.11349756250472907No Hit
GGATATTGCAACCTATTTCTGTCAGCAGTATGATAATCTCCCTCTCACTT9360.11241663333801735No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG9350.11229653009727163No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG9290.11157591065279714No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC9210.1106150847268312No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9200.11049498148608544No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAAGCGAGTCAGG9140.10977436204161098No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC9050.10869343287489926No Hit
TCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGA8620.10352899352283224No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT8470.10172744491164605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC20750.060.757574
CAACGCA21650.058.2318535
AACGCAG22750.055.9957166
ACGCAGA25450.050.3348857
CGCAGAG25900.049.7382058
GCAGAGT28750.045.55869
ATCAACG29550.043.639123
TACGCTG750.001530249338.4219325
GAATACG3600.035.9837849
GTACATG23400.032.9954341
TACATGG24050.032.6506542
GTATAGA2601.1641532E-1030.5284841
GTGCTAG4300.030.2057761
GTATATA4100.029.919271
ATACCGT1000.006294722728.788766
GTGTAGA4800.028.5626351
GTATCAA45200.028.4162981
TATCAAC45600.028.1213132
TACACGG8800.027.015425
ATACACG8800.027.015424
Produced by FastQC (version 0.11.5)