FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005280115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961133
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCGCGGCTGTCACAGAGCTCAGCCTCAGGGAGA35980.37434985584721364No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC32620.3393911144451392No Hit
GCCCCACTCTGTGTCGGCGTCTCCGGGGAAGACGGTAACCATCTCCTGCA24530.2552196210097874No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACATTGGTGCTA22350.2325380566477272No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT21090.21942852862194934No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA19950.2075675270748169No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC19940.20746348320159644No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC19490.20278150890667576No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG18760.19518630616158222No Hit
GGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTATGAGGAT17930.18655066468428405No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAC17530.18238890975546568No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17420.18124442715004063No Hit
GTCTATTACTGTGCGAGAGGCGCGTGGGAGGGGGTTTCGGGGTTCGGCCC17340.18041207616427696No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17330.1803080322910565No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA17120.17812311095342684No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17040.17729075996766316No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA16940.17625032123545856No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGCGTCTCCGGGGAAGACG16790.17468966313715167No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16450.17115217144765604No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC16400.17063195208155377No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATGAGT16160.16813489912426272No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA16080.16730254813849904No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16000.16647019715273537No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15920.1656378461669717No Hit
ACCACAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGC15820.16459740743476708No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15610.16241248609713746No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15470.16095587187205102No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT15290.15908308215408273No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15040.15648198532357124No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC14300.1487827387052572No Hit
CCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACA14160.1473261244801708No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT14030.14597355412830484No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGT13930.14493311539610024No Hit
GTGTCGGCGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCGGCAGCAG13660.14212393081914781No Hit
GTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTATGAGG13560.14108349208694324No Hit
CTCCTGCACCGGCAGCAGTGGCAACATTGCCAGCAACTTTGTGCAGTGGT13510.1405632727208409No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13510.1405632727208409No Hit
ACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGG13420.1396268778618568No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13190.1372338687777862No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG13130.13660960553846346No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA13060.13588129842592023No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13030.13556916680625886No Hit
CCACAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGCT12870.1339044648347315No Hit
CCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTT12830.13348828934184967No Hit
ATCAAAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTTAGTCCAG12610.13119932413099958No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACATTGGTGCTAT12530.13036697314523588No Hit
GACTACTACTGTCAGTCTTTTGATAGCACCAATGTGGTGTTCGGCGGAGG12500.1300548415255745No Hit
TCTCTGGACTAAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTTT12370.12870227117370853No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12350.1284941834272676No Hit
GGTGTGTCTCATGAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12300.12797396406116532No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG12050.12537286723065383No Hit
CCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGCTCCTCCAA12000.12485264786455151No Hit
CTTCTCACCCTCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCT11400.11861001547132394No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11380.11840192772488303No Hit
TCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGC11310.11767362061233981No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCTTTAGTCCAGAGATGGTGAGGGAGG11300.11756957673911936No Hit
CTCATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11080.11528061152826924No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG11060.11507252378182833No Hit
GGCCTGGGCTCCACTACTTCTCACCCTCCTCGCTCACTGCACAGGTTCTT10820.1125754708245373No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTCATGAGACACAC10800.11236738307809638No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10690.1112229004726713No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10590.11018246174046673No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC10500.10924606688148257No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG10460.10882989138860073No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC10410.10830967202249844No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT10310.10726923329029386No Hit
ACCTACTACAACCCGTCCCTCAAGAGTCGAGTCACCACATCAATAGACAC10250.1066449700509711No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG10230.10643688230453019No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10120.10529239969910512No Hit
GCCTGGAGCATCTCCACTATGGCCTGGGCTCCACTACTTCTCACCCTCCT10090.10498026807944374No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA9890.10289939061503454No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG9790.10185895188282994No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9720.10113064477028674No Hit
TCCTCGCTCACTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCC9690.10081851315062536No Hit
GATCTATGAGGATAACCACAGACCCTCTGGGGTCCCTGATCGGTTCTCTG9640.10029829378452307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)