FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences961133
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCGCGGCTGTCACAGAGCTCAGCCTCAGGGAGA34810.3621767226804199No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC30190.3141084532525676No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACATTGGTGCTA20820.2166193440449969No Hit
GCCCCACTCTGTGTCGGCGTCTCCGGGGAAGACGGTAACCATCTCCTGCA20770.2160991246788946No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAC20280.21100097489109207No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT20160.20975244841244656No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC18990.19757931524565278No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG18770.19529035003480266No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC18190.18925580538801604No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT18120.1885274982754728No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17690.18405361172699303No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA17690.18405361172699303No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA17130.1782271548266473No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATGAGT16150.16803085525104225No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15880.16522167067408985No Hit
GTCTATTACTGTGCGAGAGGCGCGTGGGAGGGGGTTTCGGGGTTCGGCCC15730.16366101257578297No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15690.16324483708290113No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15650.1628286615900193No Hit
GGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTATGAGGAT15540.16168417898459422No Hit
ACCACAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGC15200.1581466872950986No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15180.1579385995486577No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15080.15689816081645308No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14990.15596176595746894No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA14970.155753678211028No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA14790.15388088849305975No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA14560.15148787940898917No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13740.1429562818049115No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT13340.13879452687609312No Hit
ATTTTATGCTGACTCAGCCCCACTCTGTGTCGGCGTCTCCGGGGAAGACG13200.13733791265100667No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT13200.13733791265100667No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13190.1372338687777862No Hit
ATCAAAAGACTGACAGTAGTAGTCAGCCTCGTCCTCAGTCTTTAGTCCAG13170.1370257810313453No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCTTTAGTCCAGAGATGGTGAGGGAGG13130.13660960553846346No Hit
TCTCTGGACTAAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTTT12850.1336963770882906No Hit
CTCCTGCACCGGCAGCAGTGGCAACATTGCCAGCAACTTTGTGCAGTGGT12660.13171954349710185No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG12360.12859822730048806No Hit
GTGGTACCAGCAGCGCCCGGGCAGTGCCCCCACCACTGTGATCTATGAGG12350.1284941834272676No Hit
GACTACTACTGTCAGTCTTTTGATAGCACCAATGTGGTGTTCGGCGGAGG12350.1284941834272676No Hit
GGTGTGTCTCATGAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12220.12714161307540164No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC12050.12537286723065383No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACATTGGTGCTAT12020.12506073561099246No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG11990.12474860399133106No Hit
CCACAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGCT11910.12391625300556738No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11810.1228758142733628No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA11810.1228758142733628No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA11800.12277177040014232No Hit
GTGTCGGCGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCGGCAGCAG11660.12131515617505591No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11630.12100302455539452No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11470.11933832258386717No Hit
GTCTTTAGTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGAT11440.11902619096420579No Hit
CTGCACAGGTTCTTGGGCCAATTTTATGCTGACTCAGCCCCACTCTGTGT11420.11881810321776487No Hit
CTCATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10990.11434421666928511No Hit
CCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGCTCCTCCAA10830.11267951469775775No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG10720.11153503209233269No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10580.11007841786724624No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTCATGAGACACAC10320.10737327716351432No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG9680.1007144692774049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11700.070.147571
ATCAACG13350.059.249433
TCAACGC13450.057.2110024
GGTATCA4700.056.6757771
CAACGCA14050.054.7706835
TAGACGG11600.052.0789225
AGACGGC11700.051.641886
TATCAAC15650.051.001322
GACGGCC12050.050.1419077
AACGCAG15400.049.9771736
ATAGACG12650.048.890674
TAATAGA13400.047.2226832
GTAATAG13550.046.7559621
CGCAGAG17000.044.8526578
GAATACG2900.042.167929
ACGCAGA21150.036.397
GTACATG25750.036.0666731
TCGCGTA800.0021165135.9667559
GTATGTA12100.035.6992841
TACATGG25400.035.387442
Produced by FastQC (version 0.11.5)