FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1221631
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23650.19359364652665165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12650.10355009000262763No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14850.072.27071
GTATCAA30700.067.335781
ATCAACG34200.059.5778663
TCAACGC34300.059.4041674
CAACGCA35250.058.0074545
AACGCAG37950.054.070166
TATCAAC41350.049.7983672
CGCAGAG43150.047.3873188
ACGCAGA47050.043.459367
GCAGAGT48700.042.134769
GTACATG43200.032.846181
TACATGG42450.031.8863472
ACATGGG43700.029.8209653
CAGAGTA49800.024.2887159
CATGGGA19950.022.3755344
AGTACGG2303.315651E-521.9126455
CATGGGT10250.021.7752134
TACCGTC2654.015523E-621.7354537
CATGGGG27150.020.9498944
ACTACGC6500.019.9380998
Produced by FastQC (version 0.11.5)