FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1005161
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAATAACTGGCCTCCGAGACGATTCACTTTCGGCCCTGGGACCAAAG35660.3547690370000428No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT24060.2393646390976172No Hit
TTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCT19670.19569004368454407No Hit
GTTATTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGC18840.1874326600415257No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17060.16972405415649833No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15150.15072212312256444No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14310.14236525292963018No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCT13650.13579914063518184No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTC13650.13579914063518184No Hit
TTATTACTGTCAGCAGTATAATAACTGGCCTCCGAGACGATTCACTTTCG11580.11520542480259381No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT11110.11052955695654726No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11100.11043007040663139No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTG10500.10446087741167832No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10320.10267011951319241No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19700.079.3923651
GGTATCA8700.068.76131
ATCAACG23150.066.626653
TCAACGC23700.064.776354
CAACGCA25200.060.63465
TATCAAC26150.058.9830632
AACGCAG26350.058.261846
ACGCAGA30450.050.391977
CGCAGAG30750.049.8755158
GCAGAGT37350.040.8653229
GTACATG27400.032.8809741
TACATGG29150.032.143272
GACCGGT1652.508088E-630.5620947
ACATGGG29550.030.4886173
TACACGC2553.2196112E-928.2647445
CATGGGA13850.027.0606864
TTACACG2757.203198E-926.2091264
GTATAAT28550.024.7403141
GTATTAG5500.023.5882131
GTATTAA7050.023.513861
Produced by FastQC (version 0.11.5)