FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2725263
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42480.1558748641874197No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT28630.10505408101896954No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC66450.066.2051544
CAACGCA71100.061.976565
AACGCAG72600.060.596876
ATCAACG76350.057.997823
GTATCAA77800.057.6773761
ACGCAGA82050.053.6167227
CGCAGAG83150.052.907428
TATCAAC93850.046.9520762
GCAGAGT100900.043.742849
GGTATCA44950.039.739191
GTACATG71500.028.6094381
TAGACGG15100.026.7027935
GACGGCC15700.026.1404367
TACATGG74100.025.846462
ACATGGG75050.025.1360023
CAGAGTA102400.024.890929
GTAATAG21200.023.7825931
TATATCG3554.474714E-922.3105325
ATAGACG19200.021.7506564
ATACCGA2404.6045363E-521.0006336
Produced by FastQC (version 0.11.5)