FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1492952
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTGTACTGGTCATCTATTATGACAACGACCGGCCCTCAGGCATCCCG47150.3158172533343336No Hit
GTACAGGGGCCTGGCCTGGCCTCTGCTGGTACCAGTGCACACTTTCACTT44830.3002775708797068No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTG41860.28038409808218884No Hit
CCTATGTACTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACG41130.2754944566201727No Hit
GTCTCTGTGACCTCCTATGTACTGACTCAGCCACCCTCAGTGTCAGTGGC37650.25218493293823246No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31440.2105894898161495No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGTCTCTG29690.19886774658528875No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24870.16658271665800375No Hit
CACTACTACTATCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCT23910.160152503228503No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA22940.15365530840911162No Hit
GTACTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGAAAGACGGCCCG22940.15365530840911162No Hit
ATCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCTTCGACCCTGC21950.14702415080993897No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20630.13818260734437543No Hit
GTAGTAGTGAACTCCAGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTG20470.13711090510612534No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT20250.1356373145285314No Hit
CTACTATCCCACACCTGACAATAATAGTCGGCCTCATCCCCGGCTTCGAC19000.1272646407922023No Hit
GTACTGGTCATCTATTATGACAACGACCGGCCCTCAGGCATCCCGGATCG18890.12652784550340532No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18700.12525519909548333No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG18270.1223749993301861No Hit
GCCCTCAGGCATCCCGGATCGATTCTCTGGCTCCAACTCTGGGAACACGG16760.11226080945670054No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA16150.10817494467337196No Hit
GGTACCAGCAGAGGCCAGGCCAGGCCCCTGTACTGGTCATCTATTATGAC15940.10676833548566866No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15900.10650040992610613No Hit
GACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCTGGAGTTCACTA15740.105428707687856No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC15550.10415606127993399No Hit
CCTCTCTCACTGCACAGTCTCTGTGACCTCCTATGTACTGACTCAGCCAC15240.10207963819332437No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA15220.10194567541354309No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA15060.10087397317529298No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC14980.10033812205616792No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA14960.10020415927638666No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29450.072.125751
GGTATCA13100.063.209181
ATCAACG32900.061.940293
TCAACGC33450.060.9218444
TATCAAC35850.057.0442542
CAACGCA36000.056.604655
AACGCAG37650.054.3206756
CGCAGAG42300.047.8385248
ACGCAGA45700.044.752157
GCAGAGT48800.041.1631779
GAATACG3750.034.5593649
GTACATG42500.032.020381
TATTGAG17900.030.1700765
GTGCTAG5750.030.0536351
ACATGGG44600.028.2543873
TTATTGA20200.026.7357644
ATGGGAG18300.025.9693475
ATTGAGT14200.025.8638786
TACGAGT4500.025.6029594
TACATGG52950.024.6147442
Produced by FastQC (version 0.11.5)