FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177387
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT26900.2284720317108988No Hit
GTCTATATGGAATTGACCAGCCTCACATATGAAGACACGGGCATGTATTA24470.20783310840021166No Hit
GCTTAATAGTTACCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCA23940.20333161483862147No Hit
GTATTACTGTGCGAGGTATGGTCTGGCAGCAACTGGTGGTGTCAATGGGG23760.2018028057044965No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC22740.19313955394445495No Hit
CTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT18260.15508919327290008No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCTTCGGCAG16410.13937643272772673No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC15720.133515997713581No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT15590.13241185778337963No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14870.12629662124687974No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14790.12561715052060196No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14450.12272939993392146No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14370.12204992920764371No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13790.1171237664421299No Hit
ATATGGAATTGACCAGCCTCACATATGAAGACACGGGCATGTATTACTGT13630.11576482498957437No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13600.11551002346722021No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13490.11457575121858828No Hit
CAGTAATACATGCCCGTGTCTTCATATGTGAGGCTGGTCAATTCCATATA13340.11330174360681747No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCGGTGACGGTCTCCTGCA13220.11228253751740082No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13120.1114331991095536No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12910.10964958845307447No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12750.10829064700051895No Hit
GTAATACATGCCCGTGTCTTCATATGTGAGGCTGGTCAATTCCATATAGA12650.10744130859267173No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12570.10676183786639398No Hit
ATGTATTACTGTGCGAGGTATGGTCTGGCAGCAACTGGTGGTGTCAATGG12370.10506316105069957No Hit
CTCCTGATCTATGCTGCATCCACTTTGCACAGTGGGGTCCCATCAAGGTT12060.10243021198637321No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11870.10081646901146352No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21750.073.9552841
TCAACGC23750.066.742474
ATCAACG23800.066.602263
CAACGCA24500.064.6910865
AACGCAG25750.060.649536
TATCAAC27350.058.2208022
CGCAGAG29500.052.93768
ACGCAGA32100.048.6498157
GCAGAGT34600.045.1346559
CAGCGCT5350.033.6289029
GTACATG33550.033.539431
TACATGG32950.032.3630562
ACATGGG33100.030.9103223
TACACGG8700.028.953016
CATGGGA17100.028.2307194
GTACACG8900.027.5217935
GGTATCA24300.025.8247761
CAGAGTA34300.025.1775599
GTATATA6950.024.9086421
TATGTAC11200.024.4460562
Produced by FastQC (version 0.11.5)