FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177387
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT24100.204690556291177No Hit
GCTTAATAGTTACCCTCACACTTTTGGCCAGGGGACCAAGCTGGAGATCA22660.1924600832181772No Hit
GTCTATATGGAATTGACCAGCCTCACATATGAAGACACGGGCATGTATTA22380.19008193567620502No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21250.18048441166753157No Hit
GTATTACTGTGCGAGGTATGGTCTGGCAGCAACTGGTGGTGTCAATGGGG20870.1772569257177122No Hit
CTATTAAGCTGTTGACAGTAATAAGTTGCAAAATCTTCAGGCTGCAGGCT18940.1608646944462611No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15660.13300639466887268No Hit
GTGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACAGAATTCACT14340.12179512768528955No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14310.12154032616293539No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC14290.12137045848136595No Hit
GTAATACATGCCCGTGTCTTCATATGTGAGGCTGGTCAATTCCATATAGA13950.11848270789468543No Hit
CAGTAATACATGCCCGTGTCTTCATATGTGAGGCTGGTCAATTCCATATA13940.11839777405390071No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13800.11720870028291462No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13170.11185786831347722No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCCAGTCAGGGCTTCGGCAG12940.10990438997542863No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCGGTGACGGTCTCCTGCA12890.10947972077150504No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12860.10922491924915088No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12440.1056576979361926No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12200.1036192857573593No Hit
ATGTATTACTGTGCGAGGTATGGTCTGGCAGCAACTGGTGGTGTCAATGG11920.10124113821538712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15550.062.941851
ATCAACG15500.061.2303283
TCAACGC15900.059.240264
CAACGCA17300.054.4578365
AACGCAG18700.050.3807836
TATCAAC19400.048.9211352
CGCAGAG20450.046.069478
ACGCAGA22700.041.5013477
GCAGAGT24000.038.9553689
GTACATG31150.035.347961
TACATGG31550.034.1834752
ACATGGG30950.033.9169273
GTACACG10150.031.1761045
TACACGG10150.030.4675566
TATGTAC12050.026.8503442
CATGGGA17650.026.4796034
CATGGGG18050.025.8927974
GTATGTA13050.024.8160691
ACGGGGT12750.024.2545649
AGCGCTG5550.023.3246295
Produced by FastQC (version 0.11.5)