FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2103233
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTATGTATGGTACAGCAAACTACGCACAGAAGTTCCAGGACAGAGTC42330.201261581574652No Hit
GTAATAAACGGCCGTGTCCTCAAATCTCAGGTTACTCAGCTCCATGTAGG41810.19878919739277578No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33250.15808995009112162No Hit
GTTTATTACTGTGCGAGAGGCTACTATGAAAGTAGTGCTTATTACGACGG31350.14905623865734324No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT31130.148010229965011No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA29520.14035534817112513No Hit
ACATAGGGATGATCCCCCCCATCCACTCAAGCCCTTGTCCAGGGTCCTGT27640.13141672843664967No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT26100.12409466759032405No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC24780.11781861543633063No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA23890.11358703481735023No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC23700.11268366367397241No Hit
GCCTACATGGAGCTGAGTAACCTGAGATTTGAGGACACGGCCGTTTATTA22170.10540914867729824No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG21780.1035548605408911No Hit
TTCCAGGACAGAGTCACCATTACCGCGGACGATTCCACCAACACAGCCTA21290.10122511390796932No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40850.073.8512341
GGTATCA16500.067.636881
TCAACGC48100.062.7242854
ATCAACG48350.062.2510343
TATCAAC50950.058.650362
CAACGCA53400.056.0943155
AACGCAG55800.053.423576
CGCAGAG61950.047.770198
ACGCAGA67800.043.6494647
GCAGAGT73450.039.9901059
GTACATG52350.029.84551
CAGAGTA67650.029.1586179
CAGCGCT11450.024.521299
ACATGGG62350.024.3676013
GAATACG5050.024.2349289
GTCTAGC2852.9735747E-722.7370151
GTATATA12100.022.0166951
GTGCTAG7850.022.0129051
TAAACGG28500.021.9806825
AACGGCC29650.021.6138487
Produced by FastQC (version 0.11.5)