FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003009
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA42160.4203352113490507No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG35720.35612840961546705No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG34470.34366590927897955No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG29350.2926195079007267No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT28100.28015700756423917No Hit
GTGTATTACTGTGCGAGAGAGGAGGGATATTGTAGTGGTGGTAGCTGCTA26700.2661990071873732No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT24570.24496290661399847No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG21810.21744570587103407No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT20770.20707690559107644No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT19180.19122460516306433No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC19050.18992850512806964No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT18820.18763540506615595No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT14770.1472569039759364No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG14310.142670703852109No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC14260.1421722038386495No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC13130.1309061035344648No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT12680.1264196034133293No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC12670.1263199034106374No Hit
CCCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCAGGGGTCGAACCAGT12410.123727703340648No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12310.122730703313729No Hit
ATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTG12060.1202382032464315No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTC11990.11954030322758818No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT11980.11944060322489629No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC11970.11934090322220439No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11870.11834390319528539No Hit
CAGTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA11540.11505380310645268No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCTATTGGATGAGCTGGG11200.11166400301492807No Hit
CTACAATATCCCTCCTCTCTCGCACAGTAATACACAGCCGTGTCCTCGGC11100.11066700298800908No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT10920.10887240293955489No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGC10850.10817450292071157No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGC10670.10637990287225739No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGTAGCTATTGG10650.10618050286687358No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG10650.10618050286687358No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTGGTCCAGCCTGGGG10530.10498410283457078No Hit
GACCCACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCA10500.10468500282649507No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA10070.10039790271074338No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23950.045.1873171
ATCAACG25350.042.598123
TCAACGC25450.042.4307444
CAGAGCG14850.041.206879
CAACGCA26300.041.059415
AACGCAG27100.039.847326
TATCAAC27600.039.409862
GTCATAG15150.038.5747531
TAGGTGG15000.038.3951075
ATAGGTG14850.038.2981534
GGTGGTC15450.036.3448837
ACGCAGA30150.035.816337
CGCAGAG30500.035.4053238
CATAGGT16100.034.8775443
TCATAGG16550.034.8200232
GTGGTCA16100.034.4303938
AGGTGGT17450.033.4169466
GGTATCA14300.033.2995761
GAGTTAC14450.032.3834158
CAGCCGT23800.032.063149
Produced by FastQC (version 0.11.5)