FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003009
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG35440.3533368095400939No Hit
GTAATACACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA32620.32522140878097805No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG29250.2916225078738077No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG26880.2679936072358274No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT21970.21904090591410444No Hit
GTGTATTACTGTGCGAGAGAGGAGGGATATTGTAGTGGTGGTAGCTGCTA21710.21644870584411505No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT19330.19272010520344282No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT19030.18972910512268584No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC17660.17607020475389554No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT16660.16610020448470553No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC15320.1527404041239909No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG15180.15134460408630432No Hit
GATCAGCACTGAGCACAGAGGACTCACCATGGAATTGGGGCTGAGCTGGG12960.1292112034887025No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT12880.1284136034671673No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT12170.12133490327604238No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA11760.11724720316567448No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC11000.10967000296109009No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10700.10667900288033308No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC10390.10358830279688416No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT10220.10189340275112188No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10120.10089640272420287No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC10110.10079670272151098No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAG16100.047.465381
GAATACG1950.044.294559
ATAGGTG16950.043.739234
TCATAGG17000.043.6105842
CATAGGT18100.042.9485783
TAGGTGG18050.041.871235
TATAGCG700.001094832141.1306534
CCCGTTG5150.040.5316628
CCCCGTT5200.040.1419337
GTGGTCA19100.039.9462628
CAGAGCG15150.039.433849
CCGTTGA5450.036.979859
AGGTGGT22100.034.849396
ACCCCGT6200.033.6674276
GTATTAG3750.032.6824531
CACACCG1352.2505634E-531.9905075
GTATCAA15150.031.8829981
ATCAACG14400.031.4906523
TCAACGC14550.031.1660084
CAACGCA15400.030.3805965
Produced by FastQC (version 0.11.5)