FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126464
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTGACCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCA43220.38367848417703543No Hit
CTATAATACTGTTGACAGTAATAAGTTGCAAAATCTTCAGACTGCAGGCA43100.3826132037952389No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG30230.26836188284756546No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG28920.2567325720129538No Hit
GTAATACACGGCAGTGTCCTCAGCCGTTAGGCTGCTGATCTGCAGATATG25460.22601698767115505No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT24090.21385503664564515No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG23480.208439861371513No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT22650.20107167206408727No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT22580.2004502585080393No Hit
GCTCCACACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGCAG20360.18074257144480427No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG18520.1644082722572581No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT18270.16218893812851543No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT17250.15313405488324527No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC16760.1487841599909096No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15190.13484674166240554No Hit
GTGTATTACTGTGCGAGGGGGACTCGCGGGTATAGCAGCTCCCCCGGAGA13390.11886753593545821No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC13160.11682574853701495No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12880.11434009431282313No Hit
CTCCCAGGTGCAGCTGGTGCAATCTGGGTCTGAGTTGAAGAAGCCTGGGG12630.11212076018408046No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC12010.1066168115447986No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC11270.10004758252372024No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23850.052.355811
TCAACGC24900.050.5971874
CAACGCA25600.049.4948845
ATCAACG25400.049.317743
AACGCAG26850.046.9225276
TATCAAC27550.045.764842
GAATACG2800.043.7097749
GGTATCA12800.041.728131
ACGCAGA30500.041.0711677
CCCCGTT5450.040.9498987
CCGTTGA5100.040.936959
CCCGTTG5250.039.7673238
CGCAGAG31500.039.767328
CAGAGCG11450.037.7253659
TAGGTGG13400.037.0707975
ATAGGTG13750.035.0800134
GCAGAGT36500.034.7142229
ACCCCGT7100.033.4613346
GTATTAT21400.033.0536541
GGTGGTC15100.032.4204947
Produced by FastQC (version 0.11.5)