FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126464
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATAGTGACCCTCAGACGTTCGGCCAAGGGACCAAGGTGGAAATCA37870.3361847338219419No Hit
CTATAATACTGTTGACAGTAATAAGTTGCAAAATCTTCAGACTGCAGGCA34930.310085364467928No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG32900.2920643713425374No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG25040.22228850633486735No Hit
GTAATACACGGCAGTGTCCTCAGCCGTTAGGCTGCTGATCTGCAGATATG23460.20826231464121356No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG22210.19716564399750014No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT18420.16352053860576105No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT18210.16165629793761718No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT17580.15606357593318562No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC16930.15029330719845463No Hit
GCTCCACACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGCAG15860.14079455712743594No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT15430.1369773024259985No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12960.1150502812340208No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12680.11256462700982897No Hit
GTGTATTACTGTGCGAGGGGGACTCGCGGGTATAGCAGCTCCCCCGGAGA12460.11061161297653542No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG12280.1090136924038407No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT11490.1020005965570138No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGTG17550.042.2476585
TAGTGAC17000.041.4972887
TCAACGC16600.041.1962854
TATATCG700.001094407241.1343355
GTCATAG14400.040.5383951
ATCAACG17250.039.6439633
CAACGCA17650.039.561215
GTATCAA18800.039.1008641
AGTGACC18750.038.775978
TTATAGT19500.038.0228924
AACGCAG18450.037.8458186
CAGAGCG13150.037.771649
TAGGTGG15500.037.1535955
GTATTAT21100.036.888021
CTATAAT20100.036.5719531
TAATACT20750.036.079274
TATCAAC20000.035.9925422
ATAATAC20000.035.6326143
ATAGGTG15900.035.313444
TCATAGG15700.035.3047872
Produced by FastQC (version 0.11.5)