FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005280133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005280133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1248777
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC39410.3155887720545782No Hit
GTAATAATCAGCCTCATCCTCAGGCTGGAGCCCAGAGATGGTCAGGGAGG32020.2564108723975538No Hit
GATTATTACTGCTGCTCATTTGCAGGCGACAACACTTGGGTGTTCGGCGG27530.2204556938508637No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTGTCG27140.21733263825326699No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23070.1847407503501426No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23060.18466067200148625No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA22980.18402004521223564No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT21320.17072703933528566No Hit
TCTCTGGGCTCCAGCCTGAGGATGAGGCTGATTATTACTGCTGCTCATTT20860.16704343529709467No Hit
CTCCTGGACAGTCAGCCACCATCTCCTGCACTGGAACCAGCAGTGATGTT19980.15999654061533805No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA19080.15278948923626876No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC18880.15118792226314226No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18190.1456625162058558No Hit
GTGTCCGGGTCTCCTGGACAGTCAGCCACCATCTCCTGCACTGGAACCAG17380.13917616996469345No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTTTGTCTCCT16970.13589295766978413No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16880.13517225253187717No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16550.13252966702621846No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA15990.12804527950146422No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA15960.12780504445549526No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA15790.12644371252833772No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15230.1219593250035835No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTTGTCGC15120.12107846316836393No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG14900.11931673949792476No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG14890.11923666114926844No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14340.11483235197317056No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT14220.11387141178929464No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14080.11275031490810608No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14050.11251007986213711No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT13780.11034796444841634No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13660.10938702426454043No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA13490.10802569233738289No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT13420.10746514389678861No Hit
ATAATAGACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAAATACA13340.10682451710753801No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33200.059.7829631
ATCAACG35700.054.0424583
TCAACGC35750.053.966874
CAACGCA36600.052.3201565
TATCAAC38150.050.7991872
AACGCAG38300.050.373786
CGCAGAG43400.044.2884038
ACGCAGA48100.040.2601787
GCAGAGT49200.038.774779
GGTATCA16050.035.9747471
GTACATG38400.024.2460691
CAGAGTA47800.024.0968679
TATTGAG16750.024.0680965
CAGCCGT8700.023.1689999
TACATGG38350.022.7310262
TAGTACG2303.319034E-521.9098154
ACATGGG39650.021.7874573
ACAGCCG9350.021.5583218
CTAGGTC31550.021.4485033
CCTAGGT31900.020.7776372
Produced by FastQC (version 0.11.5)